Open Access

Effect of ATRA on the expression of HOXA5 gene in K562 cells and its relationship with cell cycle and apoptosis

  • Authors:
    • Wen‑Jun Liu
    • Teng Zhang
    • Qu‑Lian Guo
    • Chun‑Yan Liu
    • Yong‑Qi Bai
  • View Affiliations

  • Published online on: April 4, 2016     https://doi.org/10.3892/mmr.2016.5086
  • Pages: 4221-4228
  • Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Leukemia is the most common malignant disease in children with high incidence and mortality rates, and a poor treatment effect. The aim of the present study was to examine the changes in the expression of homeobox (Hox) A5 gene and its relationship with cell cycle and apoptosis through the intervention of human K562 myeloid leukemia cell line by all-trans retinoic acid (ATRA), to analyze the role of HOXA5 in the pathogenesis and development process of myeloid leukemia. The optimal concentration of ATRA to be used with K562 cells was determined using a cell counting kit‑8 (CCK‑8). After 24, 72 and 48 h following treatment of K562 cells with 10 µmol/l ATRA, cell cycle events and apoptosis were measured using flow cytometry. HOXA5 mRNA and protein expression in K562 cells was assessed by RT‑PCR and western blot analysis, and the relationship between HOXA5 expression and cell cycle and apoptosis was analyzed. The HOXA5 mRNA and protein expression levels were increased following treatment with ATRA in K562 cells. Apoptosis was increased significantly. The cell cycle was inhibited in G0/G1 phase. Cell proliferation was also inhibited. HOXA5 mRNA and protein expression rates positively correlated with cell apoptosis and the increased percentage and cell cycle of the G0/G1 phase. However, HOXA5 negatively correlated with the reduced percentage of S stage. In conclusion, the expression of HOXA5 in cells was increased following treatment with ATRA in K562 cells, in a time-dependent manner. Additionally, ATRA may inhibit the proliferation of K562 cells and promote apoptosis by upregulating the HOXA5 mRNA and protein expression.
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May-2016
Volume 13 Issue 5

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Liu WJ, Zhang T, Guo QL, Liu CY and Bai YQ: Effect of ATRA on the expression of HOXA5 gene in K562 cells and its relationship with cell cycle and apoptosis. Mol Med Rep 13: 4221-4228, 2016
APA
Liu, W., Zhang, T., Guo, Q., Liu, C., & Bai, Y. (2016). Effect of ATRA on the expression of HOXA5 gene in K562 cells and its relationship with cell cycle and apoptosis. Molecular Medicine Reports, 13, 4221-4228. https://doi.org/10.3892/mmr.2016.5086
MLA
Liu, W., Zhang, T., Guo, Q., Liu, C., Bai, Y."Effect of ATRA on the expression of HOXA5 gene in K562 cells and its relationship with cell cycle and apoptosis". Molecular Medicine Reports 13.5 (2016): 4221-4228.
Chicago
Liu, W., Zhang, T., Guo, Q., Liu, C., Bai, Y."Effect of ATRA on the expression of HOXA5 gene in K562 cells and its relationship with cell cycle and apoptosis". Molecular Medicine Reports 13, no. 5 (2016): 4221-4228. https://doi.org/10.3892/mmr.2016.5086