Functionally conserved effects of rapamycin exposure on zebrafish

  • Authors:
    • Ceren Sucularli
    • Huma Shehwana
    • Cem Kuscu
    • Dilay Ciglidag Dungul
    • Hilal Ozdag
    • Ozlen Konu
  • View Affiliations

  • Published online on: March 28, 2016     https://doi.org/10.3892/mmr.2016.5059
  • Pages: 4421-4430
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Mechanistic target of rapamycin (mTOR) is a conserved serine/threonine kinase important in cell proliferation, growth and protein translation. Rapamycin, a well‑known anti‑cancer agent and immunosuppressant drug, inhibits mTOR activity in different taxa including zebrafish. In the present study, the effect of rapamycin exposure on the transcriptome of a zebrafish fibroblast cell line, ZF4, was investigated. Microarray analysis demonstrated that rapamycin treatment modulated a large set of genes with varying functions including protein synthesis, assembly of mitochondrial and proteasomal machinery, cell cycle, metabolism and oxidative phosphorylation in ZF4 cells. A mild however, coordinated reduction in the expression of proteasomal and mitochondrial ribosomal subunits was detected, while the expression of numerous ribosomal subunits increased. Meta‑analysis of heterogeneous mouse rapamycin microarray datasets enabled the comparison of zebrafish and mouse pathways modulated by rapamycin, using Kyoto Encyclopedia of Genes and Genomes and Gene Ontology pathway analysis. The analyses demonstrated a high degree of functional conservation between zebrafish and mice in response to rapamycin. In addition, rapamycin treatment resulted in a marked dose‑dependent reduction in body size and pigmentation in zebrafish embryos. The present study is the first, to the best of our knowledge, to evaluate the conservation of rapamycin‑modulated functional pathways between zebrafish and mice, in addition to the dose‑dependent growth curves of zebrafish embryos upon rapamycin exposure.
View Figures
View References

Related Articles

Journal Cover

May-2016
Volume 13 Issue 5

Print ISSN: 1791-2997
Online ISSN:1791-3004

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Sucularli C, Shehwana H, Kuscu C, Dungul DC, Ozdag H and Konu O: Functionally conserved effects of rapamycin exposure on zebrafish. Mol Med Rep 13: 4421-4430, 2016.
APA
Sucularli, C., Shehwana, H., Kuscu, C., Dungul, D.C., Ozdag, H., & Konu, O. (2016). Functionally conserved effects of rapamycin exposure on zebrafish. Molecular Medicine Reports, 13, 4421-4430. https://doi.org/10.3892/mmr.2016.5059
MLA
Sucularli, C., Shehwana, H., Kuscu, C., Dungul, D. C., Ozdag, H., Konu, O."Functionally conserved effects of rapamycin exposure on zebrafish". Molecular Medicine Reports 13.5 (2016): 4421-4430.
Chicago
Sucularli, C., Shehwana, H., Kuscu, C., Dungul, D. C., Ozdag, H., Konu, O."Functionally conserved effects of rapamycin exposure on zebrafish". Molecular Medicine Reports 13, no. 5 (2016): 4421-4430. https://doi.org/10.3892/mmr.2016.5059