RUNX2 controls human IPO8 basal transcription in Saos-2 cells

  • Authors:
    • Jianjun Xiong
    • Zhihong Hu
    • Ting Wang
    • Xiaoyuan Xu
    • Jianyun Liu
    • Ping Wu
    • Xiangxin Che
    • Weidong Li
  • View Affiliations

  • Published online on: June 1, 2016     https://doi.org/10.3892/mmr.2016.5356
  • Pages: 1418-1424
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Abstract

Runt-related transcription factor 2 (RUNX2) is a vital regulatory factor that controls osteoblast-specific gene expression; however, RUNX2‑regulated genes in human mesenchymal stem cells (hMSCs) remain to be fully elucidated. In the present study, chromatin immunoprecipitation (ChIP)-on-chip analysis of RUNX2 in hMSCs demonstrated that importin 8 (IPO8) may be a novel target gene. The 5' flanking region of the IPO8 gene, which is ~3,300 bp in length, was cloned and inserted into the pGL3‑basic luciferase reporter vector. The results of dual luciferase reporter assays indicated that this segment possessed strong basal promoter activity. Furthermore, the RUNX2 binding site, which encompasses positions ‑496 to ‑501 bp, was required to achieve maximal IPO8 promoter activity in Saos‑2 human osteosarcoma cells. In addition, ChIP analysis indicated that RUNX2 uniquely binds to this specific IPO8 sequence motif. Cells with a knockdown in RUNX2 expression exhibited downregulated IPO8 transcription. Finally, synchronization of IPO8 and RUNX2 expression was observed in Saos‑2 cells cultured in osteoblast‑induction medium. Taken together, these results indicated that RUNX2 regulates IPO8 gene transcription, and may have a contributory role in osteoblast differentiation.
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August-2016
Volume 14 Issue 2

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Xiong J, Hu Z, Wang T, Xu X, Liu J, Wu P, Che X and Li W: RUNX2 controls human IPO8 basal transcription in Saos-2 cells. Mol Med Rep 14: 1418-1424, 2016
APA
Xiong, J., Hu, Z., Wang, T., Xu, X., Liu, J., Wu, P. ... Li, W. (2016). RUNX2 controls human IPO8 basal transcription in Saos-2 cells. Molecular Medicine Reports, 14, 1418-1424. https://doi.org/10.3892/mmr.2016.5356
MLA
Xiong, J., Hu, Z., Wang, T., Xu, X., Liu, J., Wu, P., Che, X., Li, W."RUNX2 controls human IPO8 basal transcription in Saos-2 cells". Molecular Medicine Reports 14.2 (2016): 1418-1424.
Chicago
Xiong, J., Hu, Z., Wang, T., Xu, X., Liu, J., Wu, P., Che, X., Li, W."RUNX2 controls human IPO8 basal transcription in Saos-2 cells". Molecular Medicine Reports 14, no. 2 (2016): 1418-1424. https://doi.org/10.3892/mmr.2016.5356