A recombinant plasmid containing CpG motifs as a novel vaccine adjuvant for immune protection against herpes simplex virus 2

  • Authors:
    • Zhuojing He
    • Juan Xu
    • Wei Tao
    • Ting Fu
    • Fang He
    • Ruxi Hu
    • Lan Jia
    • Yan Hong
  • View Affiliations

  • Published online on: June 23, 2016     https://doi.org/10.3892/mmr.2016.5439
  • Pages: 1823-1828
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Abstract

The aim of the present study was to evaluate the efficacy of a herpes simplex virus type 2 (HSV-2) DNA vaccine co‑immunized with a plasmid adjuvant containing CpG motifs. A novel eukaryotic expression plasmid vector containing kanamycin resistance gene (pcDNA3Kan) was acquired from pET‑28a(+) and pcDNA3 plasmids. A gene encoding full length HSV‑2 glycoprotein D (gD) was amplified from the pcDNA3‑gD plasmid, which was cloned into pcDNA3Kan resulting in the construction of the recombinant plasmid pcDNA3Kan‑gD (pgD). A DNA segment containing 8 CpG motifs was synthesized, and cloned into pcDNA3Kan, resulting in the recombinant plasmid pcDNA3Kan‑CpG (pCpG). Mice were co‑inoculated with pgD (used as a DNA vaccine) and pCpG (used as an adjuvant) by bilateral intramuscular injection. Mice inoculated with pgD+pCpG showed higher titers of antibodies than those inoculated with the DNA vaccine alone (P<0.05). In addition, mice inoculated with pgD+pCpG showed the highest percentage of CD4+ T cells in the blood of all the groups (P﹤0.05). Thus, the present study demonstrated that pCpG could stimulate the HSV‑2 DNA vaccine to induce a stronger cell‑mediated immune response than the DNA vaccine alone. The aim of the present study was to evaluate the efficacy of a HSV‑2 DNA vaccine (pgD) co‑immunized with a plasmid adjuvant containing CpG motifs (pCpG). Whether the pCpG would be able to stimulate the pgD to induce a stronger immune response compared with pgD alone.
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August-2016
Volume 14 Issue 2

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
He Z, Xu J, Tao W, Fu T, He F, Hu R, Jia L and Hong Y: A recombinant plasmid containing CpG motifs as a novel vaccine adjuvant for immune protection against herpes simplex virus 2. Mol Med Rep 14: 1823-1828, 2016
APA
He, Z., Xu, J., Tao, W., Fu, T., He, F., Hu, R. ... Hong, Y. (2016). A recombinant plasmid containing CpG motifs as a novel vaccine adjuvant for immune protection against herpes simplex virus 2. Molecular Medicine Reports, 14, 1823-1828. https://doi.org/10.3892/mmr.2016.5439
MLA
He, Z., Xu, J., Tao, W., Fu, T., He, F., Hu, R., Jia, L., Hong, Y."A recombinant plasmid containing CpG motifs as a novel vaccine adjuvant for immune protection against herpes simplex virus 2". Molecular Medicine Reports 14.2 (2016): 1823-1828.
Chicago
He, Z., Xu, J., Tao, W., Fu, T., He, F., Hu, R., Jia, L., Hong, Y."A recombinant plasmid containing CpG motifs as a novel vaccine adjuvant for immune protection against herpes simplex virus 2". Molecular Medicine Reports 14, no. 2 (2016): 1823-1828. https://doi.org/10.3892/mmr.2016.5439