Antioxidant effect of angiotensin (1‑7) in the protection of pancreatic β cell function

Zhang,Fen; Liu,Chang; Wang,Lei; Cao,Xi; Wang,Ying Ying; Yang,Jin Kui

doi:10.3892/mmr.2016.5514

Antioxidant effect of angiotensin (1‑7) in the protection of pancreatic β cell function

Authors:
- Fen Zhang
- Chang Liu
- Lei Wang
- Xi Cao
- Ying Ying Wang
- Jin Kui Yang
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Affiliations: Department of Endocrinology, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, P.R. China
Published online on: July 13, 2016 https://doi.org/10.3892/mmr.2016.5514
Pages: 1963-1969
Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

It is well known that the local renin-angiotensin system (RAS) is activated in the diabetic state, which results in an increase in the level of oxidative stress injury to pancreatic β cells. The angiotensin‑converting enzyme 2 (ACE2)/angiotensin (1‑7) [Ang (1‑7)]/Mas axis is a negative regulator of the classical renin‑angiotensin system. In order to investigate the antioxidant effect of Ang (1‑7) on pancreatic β cells, INS‑1 cells were cultured and oxidative stress was induced by treatment with H2O2. Glucose‑stimulated insulin secretion (GSIS), the generation of reactive oxygen species (ROS), mitochondrial membrane potential (MMP) and glucose-stimulated calcium (GSCa) responses in β cells were determined following treatment with Ang (1-7). It was observed that H2O2 significantly impaired the insulin secreting function, increased the production of ROS, and also decreased the levels of GSCa and MMP. Pre‑treatment with Ang (1‑7) alleviated these effects and treatment with A779 [antagonist of Ang (1‑7)] prevented the effects of Ang (1-7). Based on these findings, it was concluded that Ang (1‑7) can protect pancreatic β cells from oxidative injury and such protection can be blocked by its antagonist A779.

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