Dynamic metabolic profiling of urine biomarkers in rats with alcohol‑induced liver damage following treatment with Zhi‑Zi‑Da‑Huang decoction

  • Authors:
    • Li An
    • Qiaoling Lang
    • Wenbin Shen
    • Qingshui Shi
    • Fang Feng
  • View Affiliations

  • Published online on: July 11, 2016     https://doi.org/10.3892/mmr.2016.5494
  • Pages: 2093-2100
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Abstract

Alcoholic liver disease (ALD) is a leading cause of liver‑associated morbidity and mortality. Zhi‑Zi‑Da‑Huang decoction (ZZDHD), a traditional Chinese medicine formula, has been frequently used to treat or alleviate the symptoms of the various stages of ALD. To identify metabolic changes and the ZZDHD mechanism of action on ALD, potential urine biomarkers involved in the effects of ZZDHD were identified. Additionally, dynamic metabolomic profiles were systematically analyzed using nuclear magnetic resonance (NMR) spectroscopy in conjunction with statistical analysis. Alcohol administration to experimental rats disrupted multiple metabolic pathways, including methionine, gut bacterial, energy and amino acid metabolism. However, ZZDHD relieved certain effects of alcohol on the metabolism and regulated changes in potential characteristic biomarkers, including dimethylglycine, hippurate, lactate and creatine. The present study investigated time‑dependent metabolomic changes in the development of alcohol‑induced liver injury, including the effect of ZZDHD intervention. These findings elucidated important information regarding the metabolic responses to the protective effects of ZZDHD. 1H NMR‑based metabolomics method a reliable and useful tool for determining the metabolic progression of alcohol‑induced liver injury and elucidating the underlying mechanisms of the effect of traditional Chinese medicine formulas. This study also demonstrated that NMR‑based metabolomics approach is a powerful tool for understanding the molecular basis of pathogenesis and drug intervention processes.
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September-2016
Volume 14 Issue 3

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
An L, Lang Q, Shen W, Shi Q and Feng F: Dynamic metabolic profiling of urine biomarkers in rats with alcohol‑induced liver damage following treatment with Zhi‑Zi‑Da‑Huang decoction. Mol Med Rep 14: 2093-2100, 2016
APA
An, L., Lang, Q., Shen, W., Shi, Q., & Feng, F. (2016). Dynamic metabolic profiling of urine biomarkers in rats with alcohol‑induced liver damage following treatment with Zhi‑Zi‑Da‑Huang decoction. Molecular Medicine Reports, 14, 2093-2100. https://doi.org/10.3892/mmr.2016.5494
MLA
An, L., Lang, Q., Shen, W., Shi, Q., Feng, F."Dynamic metabolic profiling of urine biomarkers in rats with alcohol‑induced liver damage following treatment with Zhi‑Zi‑Da‑Huang decoction". Molecular Medicine Reports 14.3 (2016): 2093-2100.
Chicago
An, L., Lang, Q., Shen, W., Shi, Q., Feng, F."Dynamic metabolic profiling of urine biomarkers in rats with alcohol‑induced liver damage following treatment with Zhi‑Zi‑Da‑Huang decoction". Molecular Medicine Reports 14, no. 3 (2016): 2093-2100. https://doi.org/10.3892/mmr.2016.5494