In vitro and in vivo anti-Vibrio vulnificus activity of psammaplin A, a natural marine compound

Lee,Byung  Cheol; Lee,Arim; Jung,Jee  Hyung; Choi,Sang  Ho; Kim,Tae  Sung

doi:10.3892/mmr.2016.5522

In vitro and in vivo anti-Vibrio vulnificus activity of psammaplin A, a natural marine compound

Authors:
- Byung Cheol Lee
- Arim Lee
- Jee Hyung Jung
- Sang Ho Choi
- Tae Sung Kim
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Affiliations: Department of Life Sciences, College of Life Sciences and Biotechnology, Korea University, Seoul 136‑701, Republic of Korea, Department of Pharmacy, Pusan National University, Geumjeong‑gu, Busan 609‑735, Republic of Korea, National Research Laboratory of Molecular Microbiology and Toxicology, Department of Agricultural Biotechnology, Seoul National University, Seoul 151‑921, Republic of Korea
Published online on: July 18, 2016 https://doi.org/10.3892/mmr.2016.5522
Pages: 2691-2696

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Abstract

Vibrio vulnificus is known to induce severely fulminant and fatal septicemia in susceptible hosts. In the present study, the antimicrobial activity of natural marine product-derived compounds against V. vulnificus, were investigated in vitro and in vivo. Twelve pure compounds were isolated from natural marine products and their inhibitory effects on V. vulnificus-induced cytotoxicity were determined in INT‑407 cells. Among the 12 pure compounds tested, treatment with psammaplin A significantly suppressed V. vulnificus‑induced cytotoxicity in INT‑407 cells. Notably, treatment with psammaplin A (5-50 µg) had improved survival rates compared with that in the untreated mice, when the mice were infected with V. vulnificus intraperitoneally. In addition, the bacterial load of V. vulnificus in several tissues (spleen, liver and small intestine) was significantly lower in psammaplin A‑treated mice than in untreated mice. Furthermore, psammaplin A treatment significantly suppressed the growth of V. vulnificus. Taken together, these results indicate that psammaplin A may be a potential agent for the prevention and treatment of V. vulnificus infections.

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1	Ikeda T, Kanehara S, Ohtani T and Furukawa F: Endotoxin shock due to Vibrio vulnificus infection. Eur J Dermatol. 16:423–427. 2006.PubMed/NCBI
2	Powell JL, Wright AC, Wasserman SS, Hone DM and Morris JG Jr: Release of tumor necrosis factor alpha in response to Vibrio vulnificus capsular polysaccharide in vivo and in vitro models. Infect Immun. 65:3713–3718. 1997.PubMed/NCBI
3	Wright AC, Powell JL, Kaper JB and Morris JG Jr: Identification of a group 1-like capsular polysaccharide operon for Vibrio vulnificus. Infect Immun. 69:6893–6901. 2001. View Article : Google Scholar : PubMed/NCBI
4	Simpson LM and Oliver JD: Siderophore production by Vibrio vulnificus. Infect Immun. 41:644–649. 1983.PubMed/NCBI
5	Gray LD and Kreger AS: Purification and characterization of an extracellular cytolysin produced by Vibrio vulnificus. Infect Immun. 48:67–72. 1985.
6	Kim CM, Park RY, Chun HJ, Kim SY, Rhee JH and Shin SH: Vibrio vulnificus metalloprotease VvpE is essentially required for swarming. FEMS Microbiol Lett. 269:170–179. 2007. View Article : Google Scholar : PubMed/NCBI
7	Lee JH, Rho JB, Park KJ, Kim CB, Han YS, Choi SH, Lee KH and Park SJ: Role of flagellum and motility in pathogenesis of Vibrio vulnificus. Infect Immun. 72:4905–4910. 2004. View Article : Google Scholar : PubMed/NCBI
8	Gulig PA, Bourdage KL and Starks AM: Molecular pathogenesis of Vibrio vulnificus. J Microbiol. 43:118–131. 2005.PubMed/NCBI
9	Lee JH, Kim MW, Kim BS, Kim SM, Lee BC, Kim TS and Choi SH: Identification and characterization of the Vibrio vulnificus rtxA essential for cytotoxicity in vitro and virulence in mice. J Microbiol. 45:146–152. 2007.PubMed/NCBI
10	Lee BC, Lee JH, Kim MW, Kim BS, Oh MH, Kim KS, Kim TS and Choi SH: Vibrio vulnificus rtxE is important for virulence and its expression is induced by exposure to host cells. Infect Immun. 76:1509–1517. 2008. View Article : Google Scholar : PubMed/NCBI
11	Lee BC, Choi SH and Kim TS: Vibrio vulnificus RTX toxin plays an important role in the apoptotic death of human intestinal epithelial cells exposed to Vibrio vulnificus. Microbes Infect. 10:1504–1513. 2008. View Article : Google Scholar
12	Espat NJ, Auffenberg T, Abouhamze A, Baumhofer J, Moldawer LL and Howard RJ: A role for tumour necrosis factor-alpha in the increased mortality associated with Vibrio vulnificus infection in the presence of hepatic dysfunction. Ann Surg. 223:428–433. 1996. View Article : Google Scholar : PubMed/NCBI
13	Shin SH, Shin DH, Ryu PY, Chung SS and Rhee JH: Proinflammatory cytokine profile in Vibrio vulnificus septicemic patients' sera. FEMS Immunol Med Microbiol. 33:133–138. 2002. View Article : Google Scholar : PubMed/NCBI
14	Jung JH, Sim CJ and Lee CO: Cytotoxic compounds from a two-sponge association. J Nat Prod. 58:1722–1726. 1995. View Article : Google Scholar : PubMed/NCBI
15	Quiňoà E and Crew CP: Phenolic constituents of psammaplysilla. Tetrahedron Lett. 28:3229–3233. 1987. View Article : Google Scholar
16	Kim D, Lee IS, Jung JH and Yang SI: Psammaplin A, a natural bromotyrosine derivative from a sponge, possesses the antibacterial activity against methicillin-resistant Staphylococcus aureus and the DNA gyrase-inhibitory activity. Arch Pharm Res. 22:25–29. 1999. View Article : Google Scholar : PubMed/NCBI
17	Park Y, Liu Y, Hong J, Lee CO, Cho H, Kim DK, Im KS and Jung JH: New bromotyrosine derivatives from an association of two sponges, Jaspis wondoensis and Poecillastra wondoensis. J Nat Prod. 66:1495–1498. 2003. View Article : Google Scholar : PubMed/NCBI
18	Kim D, Lee IS, Jung JH, Lee CO and Choi SU: Psammaplin a natural phenolic compound, has inhibitory effect on human topoisomerase II and is cytotoxic to cancer cells. Anticancer Res. 19:4085–4090. 1999.
19	Shin J, Lee HS, Seo Y, et al: New bromotyrosine metabolites from the sponge Aplysinella rhax. Tetrahedron. 26:9071–9077. 2000. View Article : Google Scholar
20	Liu S, Fu X, Schmitz FJ and Kelly-Borges M: Psammaplysin F, a new bromotyrosine derivative from a sponge, Aplysinella sp. J Nat Prod. 60:614–615. 1997. View Article : Google Scholar : PubMed/NCBI
21	Tabudravu JN, Eijsink VG, Gooday GW, Jaspars M, Komander D, Legg M, Synstad B and van Aalten DM: Psammaplin A, a chitinase inhibitor isolated from the Fijian marine sponge Aplysinella rhax. Bioorganic Med Chem. 10:1123–1128. 2002. View Article : Google Scholar
22	Piña IC, Gautsch i JT, Wang GY, Sanders ML, Schmitz FJ, France D, Cornell-Kennon S, Sambucetti LC, Remiszewski SW, Perez LB, et al: Psammaplins from the sponge Pseudoceratina purpurea: Inhibition of both histone deacetylase and DNA methyltransferase. J Org Chem. 68:3866–3873. 2003. View Article : Google Scholar : PubMed/NCBI
23	Nicolaou KC, Hughes R, Pfefferkorn JA, Barluenga S and Roecker AJ: Combinatorial synthesis through disulfide exchange: Discovery of potent psammaplin A type antibacterial agents active against methicillin-resistant Staphylococcus aureus (MRSA). Chemistry. 7:4280–4295. 2001. View Article : Google Scholar : PubMed/NCBI
24	Nicolaou KC, Hughes R, Pfefferkorn JA and Barluenga S: Optimization and mechanistic studies of psammaplin A type antibacterial agents active against methicillin-resistant Staphylococcus aureus (MRSA). Chemistry. 7:4296–4310. 2001. View Article : Google Scholar : PubMed/NCBI
25	Zhang P, Bao B, Dong HT, Hong J, Lee HJ, Yoo ES, Bae KS and Jung JH: Anti-inflammatory sesquiterpenoids from a sponge-derived fungus Acremonium sp. J Nat Prod. 72:270–275. 2009. View Article : Google Scholar : PubMed/NCBI
26	Linkous DA and Oliver JD: Pathogenesis of Vibrio vulnificus. FEMS Microbiol Lett. 174:207–214. 1999. View Article : Google Scholar : PubMed/NCBI
27	Strom MS and Paranjpye RN: Epidemiology and pathogenesis of Vibrio vulnificus. Microbes Infect. 2:177–188. 2000. View Article : Google Scholar : PubMed/NCBI
28	Lee SJ, Jung YH, Oh SY, Song EJ, Choi SH and Han HJ: Vibrio vulnificus VvhA induces NF-κB-dependent mitochondrial cell death via lipid raft-mediated ROS production in intestinal epithelial cells. Cell Death Dis. 6:16552015. View Article : Google Scholar