Lycopene inhibits the cell proliferation and invasion of human head and neck squamous cell carcinoma

Ye,Min; Wu,Qundan; Zhang,Min; Huang,Jinbei

doi:10.3892/mmr.2016.5597

Lycopene inhibits the cell proliferation and invasion of human head and neck squamous cell carcinoma

Authors:
- Min Ye
- Qundan Wu
- Min Zhang
- Jinbei Huang
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Affiliations: Department of Biopharmacy, School of Pharmacy, Fujian Medical University, Fuzhou, Fujian 350004, P.R. China, Department of Urology, The First Affiliated Hospital of Fujian Medical University, Fuzhou, Fujian 350004, P.R. China
Published online on: August 8, 2016 https://doi.org/10.3892/mmr.2016.5597
Pages: 2953-2958
Copyright: © Ye et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Lycopene has been shown to be associated with anticancer effects in numerous tumor types. However, the underlying mechanisms of lycopene in human head and neck squamous cell carcinoma (HNSCC) remain to be determined. The present study aimed to investigate the involvement of lycopene overload and the cytotoxic effects of lycopene on HNSCC cells, and to determine the possible mechanisms involved. Treatment with lycopene at a dose of >10 µM for >24 h inhibited the growth of FaDu and Cal27 cells in a time‑ and dose‑dependent manner. The clearest increase in growth inhibition was due to the apoptotic population being significantly increased. The invasion abilities decreased with 25 µM lycopene exerting significant inhibitory effects (P<0.01). Mechanistic studies revealed that lycopene induced the upregulation of the pro‑apoptotic protein, B‑cell lymphoma‑associated X protein, and therefore, resulted in the inhibition of the protein kinase B and mitogen‑activated protein kinase signaling pathway. These data provided insights into the antitumor activity of lycopene in HNSCC cells.

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1	Jemal A, Bray F, Center MM, Ferlay J, Ward E and Forman D: Global cancer statistics. CA Cancer J Clin. 61:69–90. 2011. View Article : Google Scholar : PubMed/NCBI
2	Parkin DM, Bray F, Ferlay J and Pisani P: Global cancer statistics, 2002. CA Cancer J Clin. 55:74–108. 2005. View Article : Google Scholar : PubMed/NCBI
3	Leemans CR, Braakhuis BJ and Brakenhoff RH: The molecular biology of head and neck cancer. Nat Rev Cancer. 11:9–22. 2011. View Article : Google Scholar
4	Argiris A, Karamouzis MV, Raben D and Ferris RL: Head and neck cancer. Lancet. 371:1695–1709. 2008. View Article : Google Scholar : PubMed/NCBI
5	Tamagawa S, Beder LB, Hotomi M, Gunduz M, Yata K, Grenman R and Yamanaka N: Role of miR-200c/miR-141 in the regulation of epithelial-mesenchymal transition and migration in head and neck squamous cell carcinoma. Int J Mol Med. 33:879–886. 2014.PubMed/NCBI
6	Wang T, Chen T, Niu H, Li C, Xu C, Li Y, Huang R, Zhao J and Wu S: MicroRNA-218 inhibits the proliferation and metastasis of esophageal squamous cell carcinoma cells by targeting BMI1. Int J Mol Med. 36:93–102. 2015.PubMed/NCBI
7	Zhang YY, Lu L, Abliz G and Mijit F: Serum carotenoid, retinol and tocopherol concentrations and risk of cervical cancer among chinese women. Asian Pac J Cancer Prev. 16:2981–2986. 2015. View Article : Google Scholar : PubMed/NCBI
8	Ansari MS and Sgupta NP: A comparison of lycopene and orchidectomy vs orchidectomy alone in the management of advanced prostate cancer. BJU Int. 95:4532005. View Article : Google Scholar : PubMed/NCBI
9	Gharib A and Faezizadeh Z: In vitro anti-telomerase activity of novel lycopene-loaded nanospheres in the human leukemia cell line K562. Pharmacogn Mag. 10(Suppl 1): S157–S163. 2014. View Article : Google Scholar : PubMed/NCBI
10	Ford NA, Elsen AC, Zuniga K, Lindshield BL and Erdman JW Jr: Lycopene and apo-12′-lycopenal reduce cell proliferation and alter cell cycle progression in human prostate cancer cells. Nutr Cancer. 63:256–263. 2011. View Article : Google Scholar
11	Takeshima M, Ono M, Higuchi T, Chen C, Hara T and Nakano S: Anti-proliferative and apoptosis-inducing activity of lycopene against three subtypes of human breast cancer cell lines. Cancer Sci. 105:252–257. 2014. View Article : Google Scholar : PubMed/NCBI
12	Tang FY, Pai MH and Wang XD: Consumption of lycopene inhibits the growth and progression of colon cancer in a mouse xenograft model. J Agric Food Chem. 59:9011–9021. 2011. View Article : Google Scholar : PubMed/NCBI
13	Palozza P, Simone RE, Catalano A and Mele MC: Tomato lycopene and lung cancer prevention: From experimental to human studies. Cancers (Basel). 3:2333–2357. 2011. View Article : Google Scholar
14	Holzapfel NP, Holzapfel BM, Champ S, Feldthusen J, Clements J and Hutmacher DW: The potential role of lycopene for the prevention and therapy of prostate cancer: From molecular mechanisms to clinical evidence. Int J Mol Sci. 14:14620–14646. 2013. View Article : Google Scholar : PubMed/NCBI
15	Goldar S, Khaniani MS, Derakhshan SM and Baradaran B: Molecular mechanisms of apoptosis and roles in cancer development and treatment. Asian Pac J Cancer Prev. 16:2129–2144. 2015. View Article : Google Scholar : PubMed/NCBI
16	Hanahan D and Weinberg RA: Hallmarks of cancer: The next generation. Cell. 144:646–674. 2011. View Article : Google Scholar : PubMed/NCBI
17	Wheeler SE, Suzuki S, Thomas SM, Sen M, Leeman-Neill RJ, Chiosea SI, Kuan CT, Bigner DD, Gooding WE, Lai SY and Grandis JR: Epidermal growth factor receptor variant III mediates head and neck cancer cell invasion via STAT3 activation. Oncogene. 29:5135–5145. 2010. View Article : Google Scholar : PubMed/NCBI
18	Zuo T, Liu TM, Lan X, Weng YI, Shen R, Gu F, Huang YW, Liyanarachchi S, Deatherage DE, Hsu PY, et al: Epigenetic silencing mediated through activated PI3K/AKT signaling in breast cancer. Cancer Res. 71:1752–1762. 2011. View Article : Google Scholar : PubMed/NCBI
19	da Silva HB, Amaral EP, Nolasco EL, de Victo NC, Atique R, Jank CC, Anschau V, Zerbini LF and Correa RG: Dissecting major signaling pathways throughout the development of prostate cancer. Prostate Cancer. 2013:9206122013. View Article : Google Scholar : PubMed/NCBI
20	Shi L, Wang L, Wang B, Cretoiu SM, Wang Q, Wang X and Chen C: Regulatory mechanisms of betacellulin in CXCL8 production from lung cancer cells. J Transl Med. 12:702014. View Article : Google Scholar : PubMed/NCBI
21	Manning BD and Cantley LC: AKT/PKB signaling: Navigating downstream. Cell. 129:1261–1274. 2007. View Article : Google Scholar : PubMed/NCBI
22	Giovannucci E, Ascherio A, Rimm EB, Stampfer MJ, Colditz GA and Willett WC: Intake of carotenoids and retinol in relation to risk of prostate cancer. J Natl Cancer Inst. 87:1767–1776. 1995. View Article : Google Scholar : PubMed/NCBI
23	Wertz K: Lycopene effects contributing to prostate health. Nutr Cancer. 61:775–783. 2009. View Article : Google Scholar
24	Aras A, Khokhar AR, Qureshi MZ, Silva MF, Sobczak-Kupiec A, Pineda EA, Hechenleitner AA and Farooqi AA: Targeting cancer with nano-bullets: Curcumin, EGCG, resveratrol and quercetin on flying carpets. Asian Pac J Cancer Prev. 15:3865–3871. 2014. View Article : Google Scholar : PubMed/NCBI
25	Chan CM, Fang JY, Lin HH, Yang CY and Hung CF: Lycopene inhibits PDGF-BB-induced retinal pigment epithelial cell migration by suppression of PI3K/Akt and MAPK pathways. Biochem Biophys Res Commun. 388:172–176. 2009. View Article : Google Scholar : PubMed/NCBI
26	Elgass S, Cooper A and Chopra M: Lycopene treatment of prostate cancer cell lines inhibits adhesion and migration properties of the cells. Int J Med Sci. 11:948–954. 2014. View Article : Google Scholar : PubMed/NCBI
27	Huang CS, Shih MK, Chuang CH and Hu ML: Lycopene inhibits cell migration and invasion and upregulates Nm23-H1 in a highly invasive hepatocarcinoma, SK-Hep-1 cells. J Nutr. 135:2119–2123. 2005.PubMed/NCBI