Investigation of the association between miR‑181b, Bcl‑2 and LRIG1 in oral verrucous carcinoma

Deng,Zhi‑Yuan; Wang,Yue‑Hong; Quan,Hong‑Zhi; Liu,Ou‑Sheng; Li,Yi‑Ping; Li,Yuan; Zhu,Wu; Munnee,Krishna; Tang,Zhan‑Gui

doi:10.3892/mmr.2016.5608

Investigation of the association between miR‑181b, Bcl‑2 and LRIG1 in oral verrucous carcinoma

Authors:
- Zhi‑Yuan Deng
- Yue‑Hong Wang
- Hong‑Zhi Quan
- Ou‑Sheng Liu
- Yi‑Ping Li
- Yuan Li
- Wu Zhu
- Krishna Munnee
- Zhan‑Gui Tang
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Affiliations: Department of Oral and Maxillofacial Surgery, Xiangya School and Hospital of Stomatology, Central South University, Changsha, Hunan 410078, P.R. China, Department of Prosthodontics, Xiangya School and Hospital of Stomatology, Central South University, Changsha, Hunan 410078, P.R. China, Department of Oral and Maxillofacial Surgery, Changsha Stomatological Hospital, Changsha, Hunan 410005, P.R. China, Department of Oral and Maxillofacial Surgery, Yiyang Central Hospital, Yiyang, Hunan 413000, P.R. China
Published online on: August 9, 2016 https://doi.org/10.3892/mmr.2016.5608
Pages: 2991-2996
Copyright: © Deng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Abnormal expression of microRNAs (miRNAs) is involved in the development of and anti‑apoptotic effects in various types of human cancer. However, miRNA‑mediated regulation of oral verrucous carcinoma (OVC) remains to be elucidated. The present study aimed to investigate the expression of miR‑181b in OVC and oral squamous cell carcinoma (OSCC). The expression levels of miR‑181b were determined using reverse transcription‑quantitative polymerase chain reaction. The expression levels of B‑cell lymphoma 2 (Bcl‑2) and leucine rich repeats and immunoglobulin like domains 1 (LRIG1), were evaluated using immunohistochemical staining. The correlation between Bcl‑2 and LRIG1 expression was determined using a Pearson correlation analysis. The expression levels of miR‑181b and Bcl‑2 in OVC were significantly higher compared with normal mucosal tissue (NM); however, lower compared with the OSCC. The key target of miR‑181b was LRIG1 and it was significantly lower in OVC tissues compared with NM tissue; however this was higher when compared with OSCC tissue. The expression levels of Bcl‑2 were correlated with expression levels of LRIG1 in OVC tissues. Therefore, LRIG1 may be associated with anti‑apoptotic function in OVC tissues.

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