Polysaccharide isolated from the liquid culture broth of Inonotus obliquus suppresses invasion of B16-F10 melanoma cells via AKT/NF-κB signaling pathway

Lee,Ki  Rim; Lee,Jong  Seok; Lee,Sarah; Son,Youn  Kyoung; Kim,Ga  Ryun; Sim,Ye  Chan; Song,Jeong  Eun; Ha,Suk‑Jin; Hong,Eock  Kee

doi:10.3892/mmr.2016.5771

Polysaccharide isolated from the liquid culture broth of Inonotus obliquus suppresses invasion of B16-F10 melanoma cells via AKT/NF-κB signaling pathway

Authors:
- Ki Rim Lee
- Jong Seok Lee
- Sarah Lee
- Youn Kyoung Son
- Ga Ryun Kim
- Ye Chan Sim
- Jeong Eun Song
- Suk‑Jin Ha
- Eock Kee Hong
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Affiliations: Department of Bioengineering and Technology, College of Engineering, Kangwon National University, Chuncheon, Gangwon‑do 24341, Republic of Korea, Biological and Genetic Resources Assessment Division, National Institute of Biological Resources, Incheon 22689, Republic of Korea
Published online on: September 23, 2016 https://doi.org/10.3892/mmr.2016.5771
Pages: 4429-4435

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Abstract

A number of polysaccharides exhibit pharmacological activities. Polysaccharides derived from Inonotus obliquus (PLIO) appear to have various potential pharmacological properties, including anti‑tumor activity. However, the molecular mechanisms underlying these properties remain to be elucidated. The present study investigated the anti‑metastatic potential of PLIO and the underlying signaling pathways in B16‑F10 murine melanoma cells using the MTT colorimetric assay, in vitro migration and invasion assays, and flow cytometric and western blot analyses. PLIO inhibited the invasion of B16‑F10 cells and suppressed the expression of matrix metalloproteinases. PLIO treatment inhibited nuclear factor‑κB (NF‑κB) nuclear translocation in B16‑F10 cells. In addition, PLIO treatment inhibited the phosphorylation of c-Jun N‑terminal kinases and AKT. These results suggest that PLIO may suppress the invasion of highly metastatic melanoma cells via inhibition of the AKT/NF-κB signaling pathways.

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