Quercetin alleviates cell apoptosis and inflammation via the ER stress pathway in vascular endothelial cells cultured in high concentrations of glucosamine

  • Authors:
    • Xiaxia Cai
    • Lei Bao
    • Ye Ding
    • Xiaoqian Dai
    • Zhaofeng Zhang
    • Yong Li
  • View Affiliations

  • Published online on: December 15, 2016     https://doi.org/10.3892/mmr.2016.6054
  • Pages: 825-832
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Abstract

Glucosamine is a possible cause of vascular endothelial injury in the initial stages of atherosclerosis, through endoplasmic reticulum (ER) stress resulting in fatty streaks in the vascular wall. Quercetin is an anti‑diabetic and cardiovascular protective agent that has previously been demonstrated to reduce ER stress in human umbilical vein endothelial cells (HUVECs). The present study aimed to investigate whether quercetin prevents glucosamine‑induced apoptosis and inflammation via ER stress pathway in HUVECs. The effect of quercetin on cell viability, apoptosis, and protein expression levels of inflammatory cytokines and ER stress markers was investigated in glucosamine‑supplemented HUVECs. Quercetin was demonstrated to protect against glucosamine‑induced apoptosis, improved cell viability, and inhibited expression of pro‑inflammatory factors and endothelin‑1. Quercetin treatment also reduced the expression levels of glucose‑regulated protein 78, phosphorylated protein kinase‑like ER kinase, phosphorylated c‑Jun N‑terminal kinase and C/EBP homologous protein. In conclusion, quercetin may have auxiliary therapeutic potential against glucosamine‑induced cell apoptosis and inflammation, which may be partially due to alleviation of ER stress.
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February-2017
Volume 15 Issue 2

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Cai X, Bao L, Ding Y, Dai X, Zhang Z and Li Y: Quercetin alleviates cell apoptosis and inflammation via the ER stress pathway in vascular endothelial cells cultured in high concentrations of glucosamine. Mol Med Rep 15: 825-832, 2017
APA
Cai, X., Bao, L., Ding, Y., Dai, X., Zhang, Z., & Li, Y. (2017). Quercetin alleviates cell apoptosis and inflammation via the ER stress pathway in vascular endothelial cells cultured in high concentrations of glucosamine. Molecular Medicine Reports, 15, 825-832. https://doi.org/10.3892/mmr.2016.6054
MLA
Cai, X., Bao, L., Ding, Y., Dai, X., Zhang, Z., Li, Y."Quercetin alleviates cell apoptosis and inflammation via the ER stress pathway in vascular endothelial cells cultured in high concentrations of glucosamine". Molecular Medicine Reports 15.2 (2017): 825-832.
Chicago
Cai, X., Bao, L., Ding, Y., Dai, X., Zhang, Z., Li, Y."Quercetin alleviates cell apoptosis and inflammation via the ER stress pathway in vascular endothelial cells cultured in high concentrations of glucosamine". Molecular Medicine Reports 15, no. 2 (2017): 825-832. https://doi.org/10.3892/mmr.2016.6054