Plasma microRNA-451 as a novel hemolytic marker for β0-thalassemia/HbE disease

Leecharoenkiat,Kamonlak; Tanaka,Yuka; Harada,Yasuko; Chaichompoo,Porntip; Sarakul,Orawan; Abe,Yasunobu; Smith,Duncan  Richard; Fucharoen,Suthat; Svasti,Saovaros; Umemura,Tsukuru

doi:10.3892/mmr.2017.6326

Plasma microRNA-451 as a novel hemolytic marker for β0-thalassemia/HbE disease

Authors:
- Kamonlak Leecharoenkiat
- Yuka Tanaka
- Yasuko Harada
- Porntip Chaichompoo
- Orawan Sarakul
- Yasunobu Abe
- Duncan Richard Smith
- Suthat Fucharoen
- Saovaros Svasti
- Tsukuru Umemura
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Affiliations: Department of Clinical Microscopy, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok 10330, Thailand, Division of Medical Technology, Department of Health Sciences, Faculty of Medical Sciences, Kyushu University, Fukuoka 812‑8582, Japan, Department of Pathobiology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand, Department of Medical Technology, School of Allied Health Sciences, Walailak University, Nakhon Si Thammarat 80160, Thailand, Department of Medicine, Graduate School of Medical Sciences, Kyushu University, Fukuoka 812‑8582, Japan, Molecular Pathology Laboratory, Institute of Molecular Biosciences, Mahidol University, Salaya, Nakhon Pathom 73170, Thailand, Thalassemia Research Center, Institute of Molecular Biosciences, Mahidol University, Salaya, Nakhon Pathom 73170, Thailand
Published online on: March 15, 2017 https://doi.org/10.3892/mmr.2017.6326
Pages: 2495-2502
Copyright: © Leecharoenkiat et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

In Southeast Asia, particularly in Thailand, β0-thalassemia/hemoglobin E (HbE) disease is a common hereditary hematological disease. It is associated with pathophysiological processes, such as the intramedullary destruction of immature erythroid cells and peripheral hemolysis of mature red blood cells. MicroRNA (miR) sequences, which are short non-coding RNA that regulate gene expression in a suppressive manner, serve a crucial role in human erythropoiesis. In the present study, the plasma levels of the erythroid-expressed miRNAs, miR‑451 and miR‑155, were analyzed in 23 patients with β0-thalassemia/HbE and 16 control subjects. Reverse transcription‑quantitative polymerase chain reaction analysis revealed significantly higher levels of plasma miR‑451 and miR‑155 in β0‑thalassemia/HbE patients when compared to the control subjects. Notably, among the β0‑thalassemia/HbE patients, a significant increase in miR‑451 levels was detected in severe cases when compared with mild cases. The levels of plasma miR‑451 correlated with reticulocyte and platelet counts. The results suggest that increased plasma miR‑451 levels may be associated with the degree of hemolysis and accelerated erythropoiesis in β0‑thalassemia/HbE patients. In conclusion, miR‑451 may represent a relevant biomarker for pathological erythropoiesis associated with β0-thalassemia/HbE.

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