B(a)P adduct levels and fertility: A cross‑sectional study in a Sicilian population

Oliveri Conti,Gea; Calogero,Aldo Eugenio; Giacone,Filippo; Fiore,Maria; Barchitta,Martina; Agodi,Antonella; Ferrante,Margherita

doi:10.3892/mmr.2017.6396

B(a)P adduct levels and fertility: A cross‑sectional study in a Sicilian population

Authors:
- Gea Oliveri Conti
- Aldo Eugenio Calogero
- Filippo Giacone
- Maria Fiore
- Martina Barchitta
- Antonella Agodi
- Margherita Ferrante
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Affiliations: Environmental and Food Hygiene Laboratories, Department of Medical, Surgical Sciences and Advanced Technologies ‘G.F. Ingrassia’, University of Catania, I‑95123 Catania, Italy, Department of Clinical and Experimental Medicine, Surgical Sciences and Advanced Technologies ‘G.F. Ingrassia’, University of Catania, I‑95123 Catania, Italy, Department of Medical, Surgical Sciences and Advanced Technologies ‘G.F. Ingrassia’, University of Catania, I‑95123 Catania, Italy
Published online on: March 27, 2017 https://doi.org/10.3892/mmr.2017.6396
Pages: 3398-3404
Copyright: © Oliveri Conti et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Benzo(a)pyrene (BaP) is a carcinogenic polycyclic aromatic hydrocarbon for human tissues. Still today it is not fully investigated if BaP can affect negatively the male fertility through the BaP‑DNA adducts production. In the present study, BaP Tetrol I‑1 (TI‑1) and BaP Tetrol II‑2 (TII‑2) BaP‑DNA adducts were investigated in spermatozoa of a Sicilian male population. Semen samples from 86 volunteers in two eastern Sicilian cities (Regalbuto and Melilli) were collected. The quality of semen was evaluated in all samples according to the World Health Organization (WHO) guidelines. We analyzed BaP‑DNA adducts in extracted sperm cell DNA using the modified high‑performance liquid chromatography‑fluorescence method to detects both Tetrols. Differences between Tetrol levels were assessed by the Wilcoxon signed‑rank test and the Mann‑Whitney U test, as appropriate. Correlation between semen quality parameters and Tetrol concentrations were analyzed using the Spearman's correlation coefficient. Σ(TI‑1+TII‑2) were significantly higher in spermatozoa of volunteers from Regalbuto. Furthermore, a greater dispersion of the levels of adducts was observed in these specimens. TI‑1 adducts were higher than TII‑2 in Melilli samples (95% CI) and TII‑2 were higher than TI‑1 in Regalbuto semen samples (95% CI). A significant inverse correlation between sperm progressive motility and both TI‑1 and TII‑2 adducts was observed. The present study showed that BaP negatively affects male fertility by TI‑1 and TII‑2 DNA‑adduct production. These results suggest that DNA adducts could be used as biomarker to assess BaP exposure by air pollution. Further studies are needed to confirm if these findings could affect male fertility because of the growing impairment of this function observed in recent years.

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