Open Access

Flow control effect of necrostatin-1 on cell death of the NRK-52E renal tubular epithelial cell line

  • Authors:
    • Jialun Luo
    • Yiming Tao
    • Xinling Liang
    • Yuanhan Chen
    • Li Zhang
    • Fen Jiang
    • Shuangxin Liu
    • Zhiming Ye
    • Zhilian Li
    • Wei Shi
  • View Affiliations

  • Published online on: May 9, 2017     https://doi.org/10.3892/mmr.2017.6556
  • Pages: 57-62
  • Copyright: © Luo et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Apoptosis and necroptosis occur in renal tubular epithelial cell (RTEC) death in acute kidney injury (AKI), and may be regulated by several methods. The present study identified a protective effect of necrostatin‑1 (Nec‑1) on RTECs via a flow-control-like effect. The results established a hypoxic‑ischemic injury model of rat NRK‑52E RTECs using tumour necrosis factor‑α followed by ATP depletion with antimycin A and the pan-caspase pathway blocker, benzyloxycarbonyl-Val-Ala-Asp-fluoro-methylketone. Following pre‑treatment of cells with Nec‑1, cell organelle inflation, fragmentation inhibition and improved cell viability were observed with a parallel reduced expression of microtubule‑associated protein 1A/1B‑light chain 3‑II. Nec‑1 was involved in flow control in the process of cell injury and death. In conclusion, the present study indicated that Nec‑1 provides a protective effect and serves an important role in the prevention of AKI in an NRK‑52E cell model. Further studies will be required to fully investigate the role of Nec‑1 in the development of AKI in vivo.
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July-2017
Volume 16 Issue 1

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Luo J, Tao Y, Liang X, Chen Y, Zhang L, Jiang F, Liu S, Ye Z, Li Z, Shi W, Shi W, et al: Flow control effect of necrostatin-1 on cell death of the NRK-52E renal tubular epithelial cell line . Mol Med Rep 16: 57-62, 2017.
APA
Luo, J., Tao, Y., Liang, X., Chen, Y., Zhang, L., Jiang, F. ... Shi, W. (2017). Flow control effect of necrostatin-1 on cell death of the NRK-52E renal tubular epithelial cell line . Molecular Medicine Reports, 16, 57-62. https://doi.org/10.3892/mmr.2017.6556
MLA
Luo, J., Tao, Y., Liang, X., Chen, Y., Zhang, L., Jiang, F., Liu, S., Ye, Z., Li, Z., Shi, W."Flow control effect of necrostatin-1 on cell death of the NRK-52E renal tubular epithelial cell line ". Molecular Medicine Reports 16.1 (2017): 57-62.
Chicago
Luo, J., Tao, Y., Liang, X., Chen, Y., Zhang, L., Jiang, F., Liu, S., Ye, Z., Li, Z., Shi, W."Flow control effect of necrostatin-1 on cell death of the NRK-52E renal tubular epithelial cell line ". Molecular Medicine Reports 16, no. 1 (2017): 57-62. https://doi.org/10.3892/mmr.2017.6556