Open Access

The expression of DNA methyltransferases3A is specifically downregulated in chorionic villi of early embryo growth arrest cases

  • Authors:
    • Huating Gu
    • Jing Gao
    • Weiwei Guo
    • Yu Zhou
    • Qingnuan Kong
  • View Affiliations

  • Published online on: May 30, 2017     https://doi.org/10.3892/mmr.2017.6650
  • Pages: 591-596
  • Copyright: © Gu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The aim of the present study was to investigate the expression pattern of four DNA methyltransferases (DNMT1, DNMT3A, DNMT3B and DNMT3L) in placenta chorionic villi of early embryo growth arrest patients. Chorionic villous specimens were obtained from 40 pregnant patients diagnosed with early embryo growth arrest and 40 healthy women who underwent selective pregnancy termination. Reverse transcription‑quantitative polymerase chain reaction, immunohistochemistry and western blot analysis were performed to characterize the mRNA and protein expression of DNMTs in chorionic villous cells. It was identified, among the four DNMTs, DNMT3B presented the highest level of protein expression in both patient groups. Although the mRNA expressions of the four DNMTs were comparable, the DNMT3A protein was specifically downregulated in patients with early embryo growth arrest. Therefore, the current study suggests that an abnormal decrease in DNMT3A protein levels may be involved in the pathogenesis of early embryo growth arrest.
View Figures
View References

Related Articles

Journal Cover

July-2017
Volume 16 Issue 1

Print ISSN: 1791-2997
Online ISSN:1791-3004

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Gu H, Gao J, Guo W, Zhou Y and Kong Q: The expression of DNA methyltransferases3A is specifically downregulated in chorionic villi of early embryo growth arrest cases. Mol Med Rep 16: 591-596, 2017.
APA
Gu, H., Gao, J., Guo, W., Zhou, Y., & Kong, Q. (2017). The expression of DNA methyltransferases3A is specifically downregulated in chorionic villi of early embryo growth arrest cases. Molecular Medicine Reports, 16, 591-596. https://doi.org/10.3892/mmr.2017.6650
MLA
Gu, H., Gao, J., Guo, W., Zhou, Y., Kong, Q."The expression of DNA methyltransferases3A is specifically downregulated in chorionic villi of early embryo growth arrest cases". Molecular Medicine Reports 16.1 (2017): 591-596.
Chicago
Gu, H., Gao, J., Guo, W., Zhou, Y., Kong, Q."The expression of DNA methyltransferases3A is specifically downregulated in chorionic villi of early embryo growth arrest cases". Molecular Medicine Reports 16, no. 1 (2017): 591-596. https://doi.org/10.3892/mmr.2017.6650