Tensile strength suppresses the osteogenesis of periodontal ligament cells in inflammatory microenvironments

Sun,Chaofan; Liu,Fen; Cen,Shendan; Chen,Lijiao; Wang,Yi; Sun,Hao; Deng,Hui; Hu,Rongdang

doi:10.3892/mmr.2017.6644

Tensile strength suppresses the osteogenesis of periodontal ligament cells in inflammatory microenvironments

Authors:
- Chaofan Sun
- Fen Liu
- Shendan Cen
- Lijiao Chen
- Yi Wang
- Hao Sun
- Hui Deng
- Rongdang Hu
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Affiliations: Department of Orthodontics, School of Stomatology, Wenzhou Medical University, Wenzhou, Zhejiang 325027, P.R. China, Department of Histology and Embryology, School of Basic Medical Sciences, Wenzhou Medical University, Wenzhou, Zhejiang 325027, P.R. China, Department of Periodontics, School of Stomatology, Wenzhou Medical University, Wenzhou, Zhejiang 325027, P.R. China, Faculty of Dentistry, University of Hong Kong, Hong Kong 999077, SAR, P.R. China
Published online on: May 29, 2017 https://doi.org/10.3892/mmr.2017.6644
Pages: 666-672
Copyright: © Sun et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The present study aimed to investigate the role of orthodontic force in osteogenesis differentiation, matrix deposition and mineralization in periodontal ligament cells (PDLCs) cells in inflammatory microenvironments. The mesenchymal origin of PDLCs was confirmed by vimentin and cytokeratin staining. PDLCs were exposed to inflammatory cytokines (5 ng/ml IL‑1β and 10 ng/ml TNF‑α) and/or tensile strength (0.5 Hz, 12% elongation) for 12, 24 or 48 h. Cell proliferation and tensile strength‑induced cytokine expression were assessed by MTT assay and ELISA, respectively. Runt‑related transcription factor 2 (RUNX2) and type I collagen (COL‑I) expression were analysed by reverse transcription‑quantitative polymerase chain reaction and western blot analysis. Additionally, alkaline phosphatase activity was measured, and the mineralization profile was evaluated by alizarin red S staining. PDLCs exposed to tensile strength in inflammatory microenvironments exhibited reduced proliferation and mineralization potential. Treatment with the inflammatory cytokines IL‑1β and TNF‑α increased RUNX2 expression levels; however, decreased COL‑I expression levels, indicating that bone formation and matrix deposition involve different mechanisms in PDL tissues. Notably, RUNX2 and COL‑I expression levels were decreased in PDLCs exposed to a combination of an inflammatory environment and loading strength. The decreased osteogenic potential in an inflammatory microenvironment under tensile strength suggests that orthodontic force may amplify periodontal destruction in orthodontic patients with periodontitis.

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