Amplification by (‑)‑epigallocatechin gallate of prostaglandin F2α‑stimulated synthesis of osteoprotegerin in osteoblasts

  • Authors:
    • Go Sakai
    • Takanobu Otsuka
    • Kazuhiko Fujita
    • Shingo Kainuma
    • Gen Kuroyanagi
    • Tetsu Kawabata
    • Rie Matsushima‑Nishiwaki
    • Osamu Kozawa
    • Haruhiko Tokuda
  • View Affiliations

  • Published online on: August 24, 2017     https://doi.org/10.3892/mmr.2017.7354
  • Pages: 6376-6381
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Abstract

(‑)‑Epigallocatechin gallate (EGCG) and chlorogenic acid (CGA), major flavonoids in green tea, and coffee, respectively, are recognized as possessing potential benefits in a multitude of human health conditions, including bone disorders. We have previously demonstrated that prostaglandin F2α (PGF2α), a potent bone remodeling mediator, stimulates the synthesis of osteoprotegerin (OPG) through the activation of p44/p42 mitogen‑activated protein kinase (MAPK), p38 MAPK and stress activated protein kinase/c‑Jun N‑terminal kinase (SAPK/JNK) in osteoblast‑like MC3T3‑E1 cells. In the present study, the effects of EGCG and CGA on PGF2α‑stimulated OPG synthesis in MC3T3‑E1 cells were investigated. EGCG significantly upregulated PGF2α‑stimulated OPG release, whereas CGA did not affect OPG release. The PGF2α‑induced expression level of OPG mRNA was enhanced by EGCG. Regarding the intracellular signaling underlying the effect of EGCG, EGCG failed to affect PGF2α‑stimulated phosphorylation of p44/p42 MAPK, p38 MAPK or SAPK/JNK. EGCG by itself markedly induced the phosphorylation of p44/p42 MAP kinase for up to 10 min and the status decreased subsequently, whereas EGCG did not significantly affect the phosphorylation status of p38 MAPK or SAPK/JNK within 60 min. These results indicated that EGCG, but not CGA amplifies the PGF2α‑stimulated OPG synthesis in osteoblasts.
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November-2017
Volume 16 Issue 5

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Sakai G, Otsuka T, Fujita K, Kainuma S, Kuroyanagi G, Kawabata T, Matsushima‑Nishiwaki R, Kozawa O and Tokuda H: Amplification by (‑)‑epigallocatechin gallate of prostaglandin F2α‑stimulated synthesis of osteoprotegerin in osteoblasts. Mol Med Rep 16: 6376-6381, 2017.
APA
Sakai, G., Otsuka, T., Fujita, K., Kainuma, S., Kuroyanagi, G., Kawabata, T. ... Tokuda, H. (2017). Amplification by (‑)‑epigallocatechin gallate of prostaglandin F2α‑stimulated synthesis of osteoprotegerin in osteoblasts. Molecular Medicine Reports, 16, 6376-6381. https://doi.org/10.3892/mmr.2017.7354
MLA
Sakai, G., Otsuka, T., Fujita, K., Kainuma, S., Kuroyanagi, G., Kawabata, T., Matsushima‑Nishiwaki, R., Kozawa, O., Tokuda, H."Amplification by (‑)‑epigallocatechin gallate of prostaglandin F2α‑stimulated synthesis of osteoprotegerin in osteoblasts". Molecular Medicine Reports 16.5 (2017): 6376-6381.
Chicago
Sakai, G., Otsuka, T., Fujita, K., Kainuma, S., Kuroyanagi, G., Kawabata, T., Matsushima‑Nishiwaki, R., Kozawa, O., Tokuda, H."Amplification by (‑)‑epigallocatechin gallate of prostaglandin F2α‑stimulated synthesis of osteoprotegerin in osteoblasts". Molecular Medicine Reports 16, no. 5 (2017): 6376-6381. https://doi.org/10.3892/mmr.2017.7354