Genomic profile of oral squamous cell carcinomas with an adjacent leukoplakia or with an erythroleukoplakia that evolved after the treatment of primary tumor: A report of two cases

Ribeiro,Ilda   P.; Marques,Francisco; Barroso,Leonor; Rodrigues,Joana; Caramelo,Francisco; Melo,Joana   B.; Carreira,Isabel  M.

doi:10.3892/mmr.2017.7428

Genomic profile of oral squamous cell carcinomas with an adjacent leukoplakia or with an erythroleukoplakia that evolved after the treatment of primary tumor: A report of two cases

Authors:
- Ilda P. Ribeiro
- Francisco Marques
- Leonor Barroso
- Joana Rodrigues
- Francisco Caramelo
- Joana B. Melo
- Isabel M. Carreira
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Affiliations: Cytogenetics and Genomics Laboratory, Faculty of Medicine, University of Coimbra, 3000‑354 Coimbra, Portugal, Center of Investigation on Environment Genetics and Oncobiology, Faculty of Medicine, University of Coimbra, 3000‑354 Coimbra, Portugal, Maxillofacial Surgery Department, Coimbra Hospital and University Centre, EPE, 3000‑075 Coimbra, Portugal, Laboratory of Biostatistics and Medical Informatics, Faculty of Medicine, University of Coimbra, 3000‑354 Coimbra, Portugal
Published online on: September 5, 2017 https://doi.org/10.3892/mmr.2017.7428
Pages: 6780-6786
Copyright: © Ribeiro et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Oral leukoplakia and erythroleukoplakia are common oral potentially malignant disorders diagnosed in the oral cavity. The specific outcome of these lesions remains to be elucidated, as their malignant transformation rate exhibits great variation. The ability to predict which of those potentially malignant lesions are likely to progress to cancer would be vital to guide their future clinical management. The present study reported two patients with tongue squamous cell carcinoma: Case study 1 was diagnosed with a simultaneous leukoplakia and case study 2 developed an erythroleukoplakia following the primary tumor treatment. Whole genome copy number alterations were analyzed using array comparative genomic hybridization. The present study determined more genomic imbalances in the tissues from leukoplakia and erythroleukoplakia compared with their respective tumors. The present study also identified in tumor and potentially malignant lesions common alterations of chromosomal regions and genes, including FBXL5, UGT2B15, UGT2B28, KANSL1, GSTT1 and DUSP22, being some of these typical aberrations described in oral cancer and others are linked to chemoradioresistance. Several putative genes associated with hallmarks of malignancy that may have an important role in predicting the progression of leukoplakia and erythroleukoplakia to squamous cell carcinoma, namely gains in BNIPL, MCL1, STAG2, CSPP1 and ZNRF3 genes were also identified.

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1	Warnakulasuriya S: Lack of molecular markers to predict malignant potential of oral precancer. J Pathol. 190:407–409. 2000. View Article : Google Scholar : PubMed/NCBI
2	Vigneswaran N and Williams MD: Epidemiologic trends in head and neck cancer and aids in diagnosis. Oral Maxillofac Surg Clin North Am. 26:123–141. 2014. View Article : Google Scholar : PubMed/NCBI
3	Dionne KR, Warnakulasuriya S, Zain RB and Cheong SC: Potentially malignant disorders of the oral cavity: Current practice and future directions in the clinic and laboratory. Int J Cancer. 136:503–515. 2015.PubMed/NCBI
4	Warnakulasuriya S and Ariyawardana A: Malignant transformation of oral leukoplakia: A systematic review of observational studies. J Oral Pathol Med. 45:155–166. 2016. View Article : Google Scholar : PubMed/NCBI
5	Kobayashi T, Maruyama S, Abé T, Cheng J, Takagi R, Saito C and Saku T: Keratin 10-positive orthokeratotic dysplasia: A new leucoplakia-type precancerous entity of the oral mucosa. Histopathology. 61:910–920. 2012. View Article : Google Scholar : PubMed/NCBI
6	William WN Jr: Oral premalignant lesions: Any progress with systemic therapies? Curr Opin Oncol. 24:205–210. 2012. View Article : Google Scholar : PubMed/NCBI
7	Bouquot JE, Weiland LH and Kurland LT: Leukoplakia and carcinoma in situ synchronously associated with invasive oral/oropharyngeal carcinoma in Rochester, Minn., 1935–1984. Oral Surg Oral Med Oral Pathol. 65:199–207. 1988. View Article : Google Scholar : PubMed/NCBI
8	Gupta PC, Mehta FS, Daftary DK, Pindborg JJ, Bhonsle RB, Jalnawalla PN, Sinor PN, Pitkar VK, Murti PR, Irani RR, et al: Incidence rates of oral cancer and natural history of oral precancerous lesions in a 10-year follow-up study of Indian villagers. Community Dent Oral Epidemiol. 8:283–333. 1980. View Article : Google Scholar : PubMed/NCBI
9	Reibel J: Prognosis of oral pre-malignant lesions: Significance of clinical, histopathological, and molecular biological characteristics. Crit Rev Oral Biol Med. 14:47–62. 2003. View Article : Google Scholar : PubMed/NCBI
10	Neville BW, Dann DD, Allen CM and Bouqout JE: Patologia epitelialPatologia Oral & Maxilofacial. Guanabara Koogan Brazil; pp. 1–423. 2009
11	Califano J, Westra WH, Meininger G, Corio R, Koch WM and Sidransky D: Genetic progression and clonal relationship of recurrent premalignant head and neck lesions. Clin Cancer Res. 6:347–352. 2000.PubMed/NCBI
12	Ha PK and Califano JA: The molecular biology of mucosal field cancerization of the head and neck. Crit Rev Oral Biol Med. 14:363–369. 2003. View Article : Google Scholar : PubMed/NCBI
13	Pinto-Leite R, Carreira I, Melo J, Ferreira SI, Ribeiro I, Ferreira J, Filipe M, Bernardo C, Arantes-Rodrigues R, Oliveira P and Santos L: Genomic characterization of three urinary bladder cancer cell lines: Understanding genomic types of urinary bladder cancer. Tumour Biol. 35:4599–4617. 2014. View Article : Google Scholar : PubMed/NCBI
14	Jin C, Jin Y, Gisselsson D, Wennerberg J, Wah TS, Strömbäck B, Kwong YL and Mertens F: Molecular cytogenetic characterization of the 11q13 amplicon in head and neck squamous cell carcinoma. Cytogenet Genome Res. 115:99–106. 2006. View Article : Google Scholar : PubMed/NCBI
15	Ribeiro IP, Marques F, Caramelo F, Ferrão J, Prazeres H, Julião MJ, Rifi W, Savola S, de Melo JB, Baptista IP and Carreira IM: Genetic imbalances detected by multiplex ligation-dependent probe amplification in a cohort of patients with oral squamous cell carcinoma-the first step towards clinical personalized medicine. Tumour Biol. 35:4687–4695. 2014.PubMed/NCBI
16	Ribeiro IP, Marques F, Caramelo F, Pereira J, Patrício M, Prazeres H, Ferrão J, Julião MJ, Castelo-Branco M, de Melo JB, et al: Genetic gains and losses in oral squamous cell carcinoma: Impact on clinical management. Cell Oncol (Dordr). 37:29–39. 2014. View Article : Google Scholar : PubMed/NCBI
17	Rothschild BL, Shim AH, Ammer AG, Kelley LC, Irby KB, Head JA, Chen L, Varella-Garcia M, Sacks PG, Frederick B, et al: Cortactin overexpression regulates actin-related protein 2/3 complex activity, motility, and invasion in carcinomas with chromosome 11q13 amplification. Cancer Res. 66:8017–8025. 2006. View Article : Google Scholar : PubMed/NCBI
18	Pattje WJ, Melchers LJ, Slagter-Menkema L, Mastik MF, Schrijvers ML, Gibcus JH, Kluin PM, Hoegen-Chouvalova O, Van Der Laan BF, Roodenburg JL, et al: FADD expression is associated with regional and distant metastasis in squamous cell carcinoma of the head and neck. Histopathology. 63:263–270. 2013. View Article : Google Scholar : PubMed/NCBI
19	Freier K, Hofele C, Knoepfle K, Gross M, Devens F, Dyckhoff G, Plinkert P, Lichter P and Herold-Mende C: Cytogenetic characterization of head and neck squamous cell carcinoma cell lines as model systems for the functional analyses of tumor-associated genes. J Oral Pathol Med. 39:382–389. 2010.PubMed/NCBI
20	Ruiz C, Martins JR, Rudin F, Schneider S, Dietsche T, Fischer CA, Tornillo L, Terracciano LM, Schreiber R, Bubendorf L and Kunzelmann K: Enhanced expression of ANO1 in head and neck squamous cell carcinoma causes cell migration and correlates with poor prognosis. PLoS One. 7:e432652012. View Article : Google Scholar : PubMed/NCBI
21	Poh CF, Zhu Y, Chen E, Berean KW, Wu L, Zhang L and Rosin MP: Unique FISH patterns associated with cancer progression of oral dysplasia. J Dent Res. 91:52–57. 2012. View Article : Google Scholar : PubMed/NCBI
22	Bauer VL, Braselmann H, Henke M, Mattern D, Walch A, Unger K, Baudis M, Lassmann S, Huber R, Wienberg J, et al: Chromosomal changes characterize head and neck cancer with poor prognosis. J Mol Med (Berl). 86:1353–1365. 2008. View Article : Google Scholar : PubMed/NCBI
23	Rettori MM, de Carvalho AC, Longo AL, de Oliveira CZ, Kowalski LP, Carvalho AL and Vettore AL: TIMP3 and CCNA1 hypermethylation in HNSCC is associated with an increased incidence of second primary tumors. J Transl Med. 11:3162013. View Article : Google Scholar : PubMed/NCBI
24	Slaughter DP, Southwick HW and Smejkal W: Field cancerization in oral stratified squamous epithelium; clinical implications of multicentric origin. Cancer. 6:963–968. 1953. View Article : Google Scholar : PubMed/NCBI
25	Chen AJ, Zhou G, Juan T, Colicos SM, Cannon JP, Cabriera-Hansen M, Meyer CF, Jurecic R, Copeland NG, Gilbert DJ, et al: The dual specificity JKAP specifically activates the c-Jun N-terminal kinase pathway. J Biol Chem. 277:36592–36601. 2002. View Article : Google Scholar : PubMed/NCBI
26	Li JP, Fu YN, Chen YR and Tan TH: JNK pathway-associated phosphatase dephosphorylates focal adhesion kinase and suppresses cell migration. J Biol Chem. 285:5472–5478. 2010. View Article : Google Scholar : PubMed/NCBI
27	Gollin SM: Chromosomal alterations in squamous cell carcinomas of the head and neck: Window to the biology of disease. Head Neck. 23:238–253. 2001. View Article : Google Scholar : PubMed/NCBI
28	Skinner HD, Giri U, Yang L, Woo SH, Story MD, Pickering CR, Byers LA, Williams MD, El-Naggar A, Wang J, et al: Proteomic profiling identifies PTK2/FAK as a driver of radioresistance in HPV-negative head and neck cancer. Clin Cancer Res. 22:4643–4650. 2016. View Article : Google Scholar : PubMed/NCBI
29	van den Broek GB, Wreesmann VB, van den Brekel MW, Rasch CR, Balm AJ and Rao PH: Genetic abnormalities associated with chemoradiation resistance of head and neck squamous cell carcinoma. Clin Cancer Res. 13:4386–4391. 2007. View Article : Google Scholar : PubMed/NCBI
30	Thomas G, Hashibe M, Jacob BJ, Ramadas K, Mathew B, Sankaranarayanan R and Zhang ZF: Risk factors for multiple oral premalignant lesions. Int J Cancer. 107:285–291. 2003. View Article : Google Scholar : PubMed/NCBI