Propofol regulates the expression of TLR4 through miR‑21 in human umbilical vein endothelial cells

  • Authors:
    • Ling Ma
    • Yue Yang
    • Xuechen Sun
    • Meiling Jiang
    • Yang Ma
    • Xilun Yang
    • Zhiwei Guo
  • View Affiliations

  • Published online on: October 11, 2017     https://doi.org/10.3892/mmr.2017.7765
  • Pages:9074-9080
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Abstract

Propofol (2,6-diisopropylphenol) is one of the most commonly used intravenous anesthetics. Anesthetics can regulate the inflammatory process; however, the mechanism remains to be fully elucidated. The present study aimed to investigate whether and how propofol affects the inflammatory reaction in human umbilical vein endothelial cells (HUVECs). The expression levels of Toll‑like receptor 4 (TLR4) and cluster of differentiation 14 (CD14) were determined in HUVECs treated with propofol and lipopolysaccharide (LPS) using western blot and reverse transcription‑quantitative polymerase chain reaction analyses. In addition, whether propofol regulated the expression of TLR4 though microRNA (miR)‑21 was examined. The results showed that LPS promoted the expression levels of TLR4, CD14 and tumor necrosis factor α (TNFα), and suppressed the expression of miR‑21 in HUVECs. Propofol suppressed the expression levels of TLR4, CD14 and TNFα, and upregulated the expression of miR‑21 in a concentration‑dependent manner. miR‑21 downregulated the expression of TLR4 at the mRNA and protein levels, whereas the miR‑21 mimic reversed the effect of LPS on the expression of TLR4. In addition, the miR‑21 inhibitor inhibited the downregulatory effect of propofol on the expression of TLR4. TargetScan analysis showed that TLR4 was included in the list of targets of miR‑21. Fluorescent reporter assays showed that the miR‑21 mimic and propofol treatment reduced the fluorescence intensity in cells transfected with a reporter vector containing the wild‑type TLR4 3'‑untranslated region. Taken together, the results of the present study demonstrated that propofol regulated the expression of TLR4 in HUVECs through miR‑21.

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December 2017
Volume 16 Issue 6

Print ISSN: 1791-2997
Online ISSN:1791-3004

2016 Impact Factor: 1.692
Ranked #19/128 Medicine Research and Experimental
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APA
Ma, L., Yang, Y., Sun, X., Jiang, M., Ma, Y., Yang, X., & Guo, Z. (2017). Propofol regulates the expression of TLR4 through miR‑21 in human umbilical vein endothelial cells. Molecular Medicine Reports, 16, 9074-9080. https://doi.org/10.3892/mmr.2017.7765
MLA
Ma, L., Yang, Y., Sun, X., Jiang, M., Ma, Y., Yang, X., Guo, Z."Propofol regulates the expression of TLR4 through miR‑21 in human umbilical vein endothelial cells". Molecular Medicine Reports 16.6 (2017): 9074-9080.
Chicago
Ma, L., Yang, Y., Sun, X., Jiang, M., Ma, Y., Yang, X., Guo, Z."Propofol regulates the expression of TLR4 through miR‑21 in human umbilical vein endothelial cells". Molecular Medicine Reports 16, no. 6 (2017): 9074-9080. https://doi.org/10.3892/mmr.2017.7765