Reactive astrocytes increase the expression of P‑gp and Mrp1 via TNF‑α and NF‑κB signaling

Wang,Xueying; Huang,Shaoping; Jiang,Yongsheng; Liu,Yu; Song,Tingting; Li,Dan; Yang,Lin

doi:10.3892/mmr.2017.7939

Reactive astrocytes increase the expression of P‑gp and Mrp1 via TNF‑α and NF‑κB signaling

Authors:
- Xueying Wang
- Shaoping Huang
- Yongsheng Jiang
- Yu Liu
- Tingting Song
- Dan Li
- Lin Yang
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Affiliations: Department of Pediatrics, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710004, P.R. China
Published online on: November 2, 2017 https://doi.org/10.3892/mmr.2017.7939
Pages: 1198-1204

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Abstract

To understand multidrug resistance gene expression in reactive astrocytes, the present study involved stimulated astrocytes with tumor necrosis factor (TNF)‑α and determined gene expression by reverse transcription‑quantitative polymerase chain reaction and western blot analysis. Stimulation induced P‑glycoprotein (gp) and multidrug resistance‑associated protein (MRP0) 1 expression, which peaked by 24 h. Interestingly, the expression of P‑gp and Mrp1 correlated with cell proliferation. Therefore, reactivation of astrocytes may increase P‑gp and Mrp1 expression through TNF‑α and nuclear factor (NF)‑κB signaling. To the best of the authors' knowledge, the present study is the first to demonstrate that in vitro activated astrocytes likely induced P‑gp and Mrp1 expression by increasing NF‑κB expression. The results of the present study provide a novel insight into the mechanism of refractory epilepsy and suggest inhibiting cytokine signaling may block multidrug resistance.

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