Serum microRNA expression profiling in patients with multiple system atrophy

Kume,Kodai; Iwama,Hisakazu; Deguchi,Kazushi; Ikeda,Kazuyo; Takata,Tadayuki; Kokudo,Yohei; Kamada,Masaki; Fujikawa,Keiko; Hirose,Kayo; Masugata,Hisashi; Touge,Tetsuo; Masaki,Tsutomu

doi:10.3892/mmr.2017.7995

Serum microRNA expression profiling in patients with multiple system atrophy

Authors:
- Kodai Kume
- Hisakazu Iwama
- Kazushi Deguchi
- Kazuyo Ikeda
- Tadayuki Takata
- Yohei Kokudo
- Masaki Kamada
- Keiko Fujikawa
- Kayo Hirose
- Hisashi Masugata
- Tetsuo Touge
- Tsutomu Masaki
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Affiliations: Department of Neurology, Kagawa University Hospital, Kita‑gun, Kagawa 761‑0793, Japan, Life Science Research Center, Faculty of Medicine, Kagawa University, Kita‑gun, Kagawa 761‑0793, Japan, Department of Gastroenterology and Neurology, Faculty of Medicine, Kagawa University, Kita‑gun, Kagawa 761‑0793, Japan, Department of Intractable Neurological Research, Faculty of Medicine, Kagawa University, Kita‑gun, Kagawa 761‑0793, Japan, Department of Integrated Medicine, Faculty of Medicine, Kagawa University, Kita‑gun, Kagawa 761‑0793, Japan, Department of Health Sciences, Faculty of Medicine, Kagawa University, Kita‑gun, Kagawa 761‑0793, Japan
Published online on: November 7, 2017 https://doi.org/10.3892/mmr.2017.7995
Pages: 852-860
Copyright: © Kume et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Multiple system atrophy (MSA) is a sporadic neurodegenerative disease that is pathologically characterized by α‑synuclein positive glial cytoplasmic inclusions in oligodendrocytes. The clinical diagnosis of MSA is often challenging as there are no established biomarkers and diagnoses are now based on clinical findings alone. At present, the etiology and pathogenesis of MSA are unclear. It has been reported that dysregulation of microRNA (miRNA/miR) serves an important role in neurodegenerative disorders including Alzheimer's disease, Parkinson's disease and amyotrophic lateral sclerosis. The miRNA profile of patients with MSA remains to be established. The present study investigated the serum miRNA expression level of 10 patients with MSA, using microarray chips including 668 miRNAs. It was identified that 50 miRNAs were significantly upregulated and 17 miRNAs were significantly downregulated in the serum of the patients with MSA. The most upregulated miRNA was miR‑16, which may induce the accumulation of α‑synuclein. The target genes of some miRNAs upregulated in MSA (including miR‑17, 20a, 24, 25, 30d and 451) were associated with autophagy‑associated molecules. The present study concluded that the expression pattern of miRNAs may be a clinical biomarker for MSA and targeting these miRNAs may provide a novel treatment for MSA.

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