Open Access

Cornin protects SH‑SY5Y cells against oxygen and glucose deprivation‑induced autophagy through the PI3K/Akt/mTOR pathway

  • Authors:
    • Changling Ding
    • Jie Zhang
    • Baoyuan Li
    • Zhaoxing Ding
    • Wenna Cheng
    • Fei Gao
    • Ye Zhang
    • Yangyang Xu
    • Shuping Zhang
  • View Affiliations

  • Published online on: October 25, 2017     https://doi.org/10.3892/mmr.2017.7864
  • Pages:87-92
  • Copyright: © Ding et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

It has been reported that cornin may reduce neuronal death during cerebral ischemia; however, little is known about the molecular mechanism of the role of corninin autophagy in SH‑SY5Y neuronal cells. In the present study, oxygen‑glucose deprivation (OGD)‑treated cells were used as a cerebral ischemia model in vitro. The results demonstrated that cornin was able to reduce neuronal cell loss, increase the apoptosis regulator Bcl‑2/apoptosis regulator BAX ratio, and decrease the protein levels of caspase‑3. In addition, cornin decreased the microtubule‑associated proteins 1A/1B light chain 3B (LC3)‑II/LC3‑I ratio and beclin‑1 protein expression, and resulted in an upregulation in phosphorylated (p)‑RAC‑α serine/threonine‑protein kinase (Akt), p‑protein kinase mTOR (mTOR) in OGD‑treated SH‑SY5Y cells. Additionally, it was observed that following inhibition of PI3K/Akt by LY294002, the levels of p‑Akt and p‑mTOR were markedly decreased, and the LC3‑II/LC3‑I ratio and beclin‑1 were increased. Similarly, following inhibition of mTOR by rapamycin, LC3‑II/LC3‑I and Beclin‑1 were significantly increased in SH‑SY5Y cells. These results indicated that cornin protected SH‑SY5Y cells against OGD‑induced autophagy through the PI3K/Akt/mTOR pathway.

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January 2018
Volume 17 Issue 1

Print ISSN: 1791-2997
Online ISSN:1791-3004

2016 Impact Factor: 1.692
Ranked #19/128 Medicine Research and Experimental
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APA
Ding, C., Zhang, J., Li, B., Ding, Z., Cheng, W., Gao, F. ... Zhang, S. (2018). Cornin protects SH‑SY5Y cells against oxygen and glucose deprivation‑induced autophagy through the PI3K/Akt/mTOR pathway. Molecular Medicine Reports, 17, 87-92. https://doi.org/10.3892/mmr.2017.7864
MLA
Ding, C., Zhang, J., Li, B., Ding, Z., Cheng, W., Gao, F., Zhang, Y., Xu, Y., Zhang, S."Cornin protects SH‑SY5Y cells against oxygen and glucose deprivation‑induced autophagy through the PI3K/Akt/mTOR pathway". Molecular Medicine Reports 17.1 (2018): 87-92.
Chicago
Ding, C., Zhang, J., Li, B., Ding, Z., Cheng, W., Gao, F., Zhang, Y., Xu, Y., Zhang, S."Cornin protects SH‑SY5Y cells against oxygen and glucose deprivation‑induced autophagy through the PI3K/Akt/mTOR pathway". Molecular Medicine Reports 17, no. 1 (2018): 87-92. https://doi.org/10.3892/mmr.2017.7864