Open Access

Quantitative proteomic study of the plasma reveals acute phase response and LXR/RXR and FXR/RXR activation in the chronic unpredictable mild stress mouse model of depression

  • Authors:
    • Chuangchuang Yang
    • Chanjuan Zhou
    • Jie Li
    • Zhi Chen
    • Haiyang Shi
    • Wensong Yang
    • Yinhua Qin
    • Lin Lü
    • Libo Zhao
    • Liang Fang
    • Haiyang Wang
    • Zicheng Hu
    • Peng Xie
  • View Affiliations

  • Published online on: October 20, 2017     https://doi.org/10.3892/mmr.2017.7855
  • Pages:93-102
  • Copyright: © Yang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: HTML 0 views | PDF 0 views
0

Abstract

Major depressive disorder is a severe neuropsychiatric disease that negatively impacts the quality of life of a large portion of the population. However, the molecular mechanisms underlying depression are still unclear. The pathogenesis of depression involves several brain regions. However, most previous studies have focused only on one specific brain region. Plasma and brain tissues exchange numerous components through the blood‑brain barrier. Therefore, in the present study, plasma samples from control (CON) mice and mice subjected to chronic unpredictable mild stress (CUMS) were used to investigate the molecular pathogenesis of depression, and the association between the peripheral circulation and the central nervous system. A total of 47 significant differentially expressed proteins were identified between the CUMS and CON group by an isobaric tag for relative and absolute quantitation (iTRAQ) coupled with tandem mass spectrometry approach. These 47 differentially expressed proteins were analyzed with ingenuity pathway analysis (IPA) software. This revealed that the acute phase response, LXR/RXR and FXR/RXR activation, the complement system and the intrinsic prothrombin activation pathway were significantly changed. Four of the significant differentially expressed proteins (lipopolysaccharide binding protein, fibrinogen β chain, α‑1 antitrypsin, and complement factor H) were validated by western blotting. the present findings provide a novel insight into the molecular pathogenesis of depression.

Related Articles

Journal Cover

January 2018
Volume 17 Issue 1

Print ISSN: 1791-2997
Online ISSN:1791-3004

2016 Impact Factor: 1.692
Ranked #19/128 Medicine Research and Experimental
(total number of cites)

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
APA
Yang, C., Zhou, C., Li, J., Chen, Z., Shi, H., Yang, W. ... Xie, P. (2018). Quantitative proteomic study of the plasma reveals acute phase response and LXR/RXR and FXR/RXR activation in the chronic unpredictable mild stress mouse model of depression. Molecular Medicine Reports, 17, 93-102. https://doi.org/10.3892/mmr.2017.7855
MLA
Yang, C., Zhou, C., Li, J., Chen, Z., Shi, H., Yang, W., Qin, Y., Lü, L., Zhao, L., Fang, L., Wang, H., Hu, Z., Xie, P."Quantitative proteomic study of the plasma reveals acute phase response and LXR/RXR and FXR/RXR activation in the chronic unpredictable mild stress mouse model of depression". Molecular Medicine Reports 17.1 (2018): 93-102.
Chicago
Yang, C., Zhou, C., Li, J., Chen, Z., Shi, H., Yang, W., Qin, Y., Lü, L., Zhao, L., Fang, L., Wang, H., Hu, Z., Xie, P."Quantitative proteomic study of the plasma reveals acute phase response and LXR/RXR and FXR/RXR activation in the chronic unpredictable mild stress mouse model of depression". Molecular Medicine Reports 17, no. 1 (2018): 93-102. https://doi.org/10.3892/mmr.2017.7855