Long non-coding RNA TCF7 predicts the progression and facilitates the growth and metastasis of colorectal cancer

Jin,Fu‑Shu; Wang,Hong‑Mei; Song,Xiao‑Yan

doi:10.3892/mmr.2018.8708

Long non-coding RNA TCF7 predicts the progression and facilitates the growth and metastasis of colorectal cancer

Authors:
- Fu‑Shu Jin
- Hong‑Mei Wang
- Xiao‑Yan Song
View Affiliations

Affiliations: Anorectal Section, Yantai City Hospital of Traditional Chinese Medicine, Yantai, Shandong 264000, P.R. China, Department of Obstetrics and Gynecology, Yantai City Hospital of Traditional Chinese Medicine, Yantai, Shandong 264000, P.R. China, Department of Emergency, Yantai City Hospital of Traditional Chinese Medicine, Yantai, Shandong 264000, P.R. China
Published online on: March 9, 2018 https://doi.org/10.3892/mmr.2018.8708
Pages: 6902-6908

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

Long non-coding RNA (lnc)TCF7 has been reported to promote the self‑renewal of human cancer stem cells, and enhance the aggressiveness of human non‑small cell lung cancer and hepatocellular carcinoma cells. However, the effect of lncTCF7 on colorectal cancer (CRC) tumorigenesis and progression is currently unclear. In the present study, reverse transcription‑quantitative polymerase chain reaction results demonstrated that lncTCF7 expression was higher in CRC tissues compared with adjacent normal tissues and was significantly associated with tumor size, differentiation degree, tumor‑node‑metastasis grade, lymph node metastasis and invasion depth. In addition, lncTCF7 demonstrated a high sensitivity and specificity for diagnosing CRC, as determined by receiver operating characteristic curve analysis. Furthermore, lncTCF7 silencing in SW‑620 and HT29 CRC cell lines inhibited the proliferation, cell cycle, migration and invasion of cells, as determined by Cell Counting Kit‑8 assays, propidium iodide (PI) staining and flow cytometry, wound healing assays and Transwell invasion assays, respectively; however, Annexin V/PI double staining and flow cytometry indicated that lncTCF7 silencing did not significantly affect the apoptosis of CRC cells. These results indicate that lncTCF7 may predict the progression, and promote the growth and metastasis, of CRC, and may therefore be a novel diagnostic marker and therapeutic target for CRC treatment.

View Figures

View References

1	Torre LA, Bray F, Siegel RL, Ferlay J, Lortet-Tieulent J and Jemal A: Global cancer statistics, 2012. CA Cancer J Clin. 65:87–108. 2015. View Article : Google Scholar : PubMed/NCBI
2	Chen W, Zheng R, Zeng H, Zhang S and He J: Annual report on status of cancer in China, 2011. Chin J Cancer Res. 27:2–12. 2015. View Article : Google Scholar : PubMed/NCBI
3	Fleshman JW and Smallwood N: Current concepts in rectal cancer. Clin Colon Rectal Surg. 28:5–11. 2015. View Article : Google Scholar : PubMed/NCBI
4	Rao AKDM, Rajkumar T and Mani S: Perspectives of long non-coding RNAs in cancer. Mol Biol Rep. 44:203–218. 2017. View Article : Google Scholar : PubMed/NCBI
5	Li M, Wang Y, Cheng L, Niu W, Zhao G, Raju JK, Huo J, Wu B, Yin B, Song Y and Bu R: Long non-coding RNAs in renal cell carcinoma: A systematic review and clinical implications. Oncotarget. 8:48424–48435. 2017.PubMed/NCBI
6	Klingenberg M, Matsuda A, Diederichs S and Patel T: Non-coding RNA in hepatocellular carcinoma: Mechanisms, biomarkers and therapeutic targets. J Hepatol. 67:603–618. 2017. View Article : Google Scholar : PubMed/NCBI
7	Richtig G, Ehall B, Richtig E, Aigelsreiter A, Gutschner T and Pichler M: Function and clinical implications of long non-coding RNAs in melanoma. Int J Mol Sci. 18:pii: E715. 2017. View Article : Google Scholar : PubMed/NCBI
8	Yang Y, Zhao L, Lei L, Lau WB, Lau B, Yang Q, Le X, Yang H, Wang C, Luo Z, et al: LncRNAs: The bridge linking RNA and colorectal cancer. Oncotarget. 8:12517–12532. 2017.PubMed/NCBI
9	Wang Y, He L, Du Y, Zhu P, Huang G, Luo J, Yan X, Ye B, Li C, Xia P, et al: The long noncoding RNA lncTCF7 promotes self-renewal of human liver cancer stem cells through activation of Wnt signaling. Cell Stem Cell. 16:413–425. 2015. View Article : Google Scholar : PubMed/NCBI
10	Weber BN, Chi AW, Chavez A, Yashiro-Ohtani Y, Yang Q, Shestova O and Bhandoola A: A critical role for TCF-1 in T-lineage specification and differentiation. Nature. 476:63–68. 2011. View Article : Google Scholar : PubMed/NCBI
11	Cui L, Guan Y, Qu Z, Zhang J, Liao B, Ma B, Qian J, Li D, Li W, Xu GT and Jin Y: WNT signaling determines tumorigenicity and function of ESC-derived retinal progenitors. J Clin Invest. 123:1647–1661. 2013. View Article : Google Scholar : PubMed/NCBI
12	Nikuseva-Martic T, Serman L, Zeljko M, Vidas Z, Gašparov S, Zeljko HM, Kosović M and Pećina-Šlaus N: Expression of secreted frizzled-related protein 1 and 3, T-cell factor 1 and lymphoid enhancer factor 1 in clear cell renal cell carcinoma. Pathol Oncol Res. 19:545–551. 2013. View Article : Google Scholar : PubMed/NCBI
13	Siu MK, Chen WY, Tsai HY, Chen HY, Yin JJ, Chen CL, Tsai YC and Liu YN: TCF7 is suppressed by the androgen receptor via microRNA-1-mediated downregulation and is involved in the development of resistance to androgen deprivation in prostate cancer. Prostate Cancer Prostatic Dis. 20:172–178. 2017. View Article : Google Scholar : PubMed/NCBI
14	Chen WY, Liu SY, Chang YS, Yin JJ, Yeh HL, Mouhieddine TH, Hadadeh O, Abou-Kheir W and Liu YN: MicroRNA-34a regulates WNT/TCF7 signaling and inhibits bone metastasis in Ras-activated prostate cancer. Oncotarget. 6:441–457. 2015.PubMed/NCBI
15	Wang Y, Zhang S, Xu Y, Zhang Y, Guan H, Li X, Li Y and Wang Y: Upregulation of miR-192 inhibits cell growth and invasion and induces cell apoptosis by targeting TCF7 in human osteosarcoma. Tumour Biol. 37:15211–15220. 2016. View Article : Google Scholar : PubMed/NCBI
16	Wu J and Wang D: Long noncoding RNA TCF7 promotes invasiveness and self-renewal of human non-small cell lung cancer cells. Hum Cell. 30:23–29. 2017. View Article : Google Scholar : PubMed/NCBI
17	Wu J, Zhang J, Shen B, Yin K, Xu J, Gao W and Zhang L: Long noncoding RNA lncTCF7, induced by IL-6/STAT3 transactivation, promotes hepatocellular carcinoma aggressiveness through epithelial-mesenchymal transition. J Exp Clin Cancer Res. 34:1162015. View Article : Google Scholar : PubMed/NCBI
18	Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI
19	Li C, Liang G, Yang S, Sui J, Yao W, Shen X, Zhang Y, Peng H, Hong W, Xu S, et al: Dysregulated lncRNA-UCA1 contributes to the progression of gastric cancer through regulation of the PI3K-Akt-mTOR signaling pathway. Oncotarget. 8:93476–93491. 2017.PubMed/NCBI
20	Xu C, Sun L, Jiang C, Zhou H, Gu L, Liu Y and Xu Q: SPP1, analyzed by bioinformatics methods, promotes the metastasis in colorectal cancer by activating EMT pathway. Biomed Pharmacother. 91:1167–1177. 2017. View Article : Google Scholar : PubMed/NCBI
21	Pan Y, Jiao G, Wang C, Yang J and Yang W: MicroRNA-421 inhibits breast cancer metastasis by targeting metastasis associated 1. Biomed Pharmacother. 83:1398–1406. 2016. View Article : Google Scholar : PubMed/NCBI
22	Romagnoli P, Filipponi F, Bandettini L and Brugnola D: Increase of mitotic activity in the colonic mucosa of patients with colorectal cancer. Dis Colon Rectum. 27:305–308. 1984. View Article : Google Scholar : PubMed/NCBI
23	Paganelli GM, Biasco G, Santucci R, Brandi G, Lalli AA, Miglioli M and Barbara L: Rectal cell proliferation and colorectal cancer risk level in patients with nonfamilial adenomatous polyps of the large bowel. Cancer. 68:2451–2454. 1991. View Article : Google Scholar : PubMed/NCBI
24	Yao M, Lam EC, Kelly CR, Zhou W and Wolfe MM: Cyclooxygenase-2 selective inhibition with NS-398 suppresses proliferation and invasiveness and delays liver metastasis in colorectal cancer. Br J Cancer. 90:712–719. 2004. View Article : Google Scholar : PubMed/NCBI