Open Access

KCC2-GABAA pathway correlates with the analgesic effect of electro-acupuncture in CCI rats

  • Authors:
    • Si‑Si Li
    • Wen‑Zhan Tu
    • Cheng‑Qian Jia
    • Xia Jiang
    • Xin‑Ru Qian
    • Guan‑Hu Yang
    • Qi‑Miao Hu
    • Wen‑Ci Chen
    • Bin Lu
    • Song‑He Jiang
  • View Affiliations

  • Published online on: March 16, 2018     https://doi.org/10.3892/mmr.2018.8766
  • Pages: 6961-6968
  • Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Potassium-chloride cotransporter 2 (KCC2) has been indicated to serve a crucial role during chronic neuropathic pain (NP). Following the emergence of NP, γ‑aminobutyric acid (GABA) A receptor‑mediated signaling may be further impaired by the changes of KCC2 chloride anion gradient. In the present study, the authors investigate the effect of electro-acupuncture (EA) on the behavior and the expression of KCC2 and GABAA receptor γ2 subunit in the spinal cord of chronic constriction injury (CCI) model rats. A total of 60 adult male Sprague‑Dawley rats were divided into four groups: Normal group, sham‑CCI group, CCI group and CCI+EA group. The effect of EA was assessed via the values of mechanical withdrawal threshold and thermal withdrawal latency, which were significantly improved upon stimulation of the ST‑36 and GB‑34 acupoints. In addition, a marked reduction in both the mRNA and protein levels of KCC2 and GABAA receptor γ2 subunit was observed in the spinal cord following loose ligation of the sciatic nerve. The reductions in KCC2 and GABAA receptor γ2 subunit expression were reversed by EA treatment. These results support the notion that KCC2 and GABAA receptor γ2 subunit contribute to NP following peripheral nerve injury and extend the understanding of the analgesic effects of EA on NP.
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May-2018
Volume 17 Issue 5

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Li SS, Tu WZ, Jia CQ, Jiang X, Qian XR, Yang GH, Hu QM, Chen WC, Lu B, Jiang SH, Jiang SH, et al: KCC2-GABAA pathway correlates with the analgesic effect of electro-acupuncture in CCI rats. Mol Med Rep 17: 6961-6968, 2018
APA
Li, S., Tu, W., Jia, C., Jiang, X., Qian, X., Yang, G. ... Jiang, S. (2018). KCC2-GABAA pathway correlates with the analgesic effect of electro-acupuncture in CCI rats. Molecular Medicine Reports, 17, 6961-6968. https://doi.org/10.3892/mmr.2018.8766
MLA
Li, S., Tu, W., Jia, C., Jiang, X., Qian, X., Yang, G., Hu, Q., Chen, W., Lu, B., Jiang, S."KCC2-GABAA pathway correlates with the analgesic effect of electro-acupuncture in CCI rats". Molecular Medicine Reports 17.5 (2018): 6961-6968.
Chicago
Li, S., Tu, W., Jia, C., Jiang, X., Qian, X., Yang, G., Hu, Q., Chen, W., Lu, B., Jiang, S."KCC2-GABAA pathway correlates with the analgesic effect of electro-acupuncture in CCI rats". Molecular Medicine Reports 17, no. 5 (2018): 6961-6968. https://doi.org/10.3892/mmr.2018.8766