Cell‑specific and roasting‑dependent regulation of the Keap1/Nrf2 pathway by coffee extracts

Priftis,Alexandros; Angeli‑Terzidou,Antonia‑Eugenia; Veskoukis,Aristidis  S.; Spandidos,Demetrios  A.; Kouretas,Dimitrios

doi:10.3892/mmr.2018.8924

Cell‑specific and roasting‑dependent regulation of the Keap1/Nrf2 pathway by coffee extracts

Authors:
- Alexandros Priftis
- Antonia‑Eugenia Angeli‑Terzidou
- Aristidis S. Veskoukis
- Demetrios A. Spandidos
- Dimitrios Kouretas
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Affiliations: Department of Biochemistry and Biotechnology, University of Thessaly, 41500 Larissa, Greece, Laboratory of Clinical Virology, University of Crete, Medical School, 71409 Heraklion, Crete, Greece
Published online on: April 24, 2018 https://doi.org/10.3892/mmr.2018.8924
Pages: 8325-8331
Copyright: © Priftis et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Coffee is a popular beverage that contains various bioactive compounds. However, its molecular mechanism of action is not fully elucidated. In this context, two previously characterized coffee extracts, a lightly roasted and the corresponding green one, were investigated for their effect on nuclear factor erythroid 2‑related factor 2 (Nrf2) target gene expression in myoblasts and endothelial cells using quantitative PCR. The tested concentrations were non‑cytotoxic and led to improved redox cell status, as was evident by increased reduced glutathione (GSH) levels. In both cell lines, the roasted extract upregulated gene expression more readily than its green counterpart leading to increased NAD(P)H quinone dehydrogenase 1 and γ‑glutamyl cysteine ligase catalytic subunit, among others. The green extract had a mixed effect on the endothelial cells, while, as regards the myoblasts it caused the downregulation of some Nrf‑target genes. Therefore, a potential dose‑ and roasting‑dependent mechanism is proposed in the current study, accounting for coffee's antioxidant activity.

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