Dishevelled‑2 modulates osteogenic differentiation of human synovial fibroblasts in osteoarthritis

Zhang,Lihua; Luan,Luan; Ma,Yingying

doi:10.3892/mmr.2018.8975

Dishevelled‑2 modulates osteogenic differentiation of human synovial fibroblasts in osteoarthritis

Authors:
- Lihua Zhang
- Luan Luan
- Yingying Ma
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Affiliations: Department of Rheumatology, Jining No. 1 People's Hospital, Jining, Shandong 272000, P.R. China
Published online on: May 4, 2018 https://doi.org/10.3892/mmr.2018.8975
Pages: 292-298
Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Dishevelled (Dvl)‑2 represents one of the cytoplasmic proteins, which serves as a pivotal hub in signaling intermediates through a number of different signaling pathways associated with the Wnt family. The aim of the present study was to investigate the roles and mechanisms of Dvl‑2 on synovial fibroblasts (SFBs) in osteoarthritis (OA). A Cell Counting kit‑8 (CCK‑8) assay was used to determine cell viability. An alkaline phosphatase (ALP) test kit was used to measure the activity of ALP. Western blot and reverse transcription‑quantitative polymerase chain reaction analysis were used to evaluate the protein and mRNA expression, respectively. The results suggest that depletion of Dvl‑2 significantly decreased the expression of osteoprotegerin (OPG) and ALP (P<0.05) and significantly increased the expression of receptor activator of nuclear factor‑κB ligand (RANKL), ALP, osteonectin (ON), osteocalcin (OCN) and osterix (P<0.05). In addition, the depletion of Dvl‑2 also significantly inhibited the expression of runt‑related transcription factor 2 (Runx‑2) and β‑catenin in SFBs (P<0.05). The effect of Dvl‑2 over‑expression was opposite to the effect of Dvl‑2 silencing. The inactivation of Wnt3a reversed the effect of Dvl‑2 silencing. In conclusion, the results indicate that Dvl‑2 regulated osteogenic differentiation of SFBs in OA.

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