Open Access

Platelet‑rich fibrin exudate promotes the proliferation and osteogenic differentiation of human periodontal ligament cells in vitro

  • Authors:
    • Xiaoju Li
    • Huixiao Yang
    • Zijiian Zhang
    • Zhonghai Yan
    • Huling Lv
    • Yan Zhang
    • Bin Wu
  • View Affiliations

  • Published online on: September 10, 2018     https://doi.org/10.3892/mmr.2018.9472
  • Pages: 4477-4485
  • Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 4.0].

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Abstract

The purpose of the present study was to evaluate the effects of platelet‑rich fibrin (PRF) exudate on the proliferation, osteogenic differentiation and mineralization of human periodontal ligament cells (hPDLCs) in vitro. In the present study PRF was obtained with permission, from the peripheral blood of healthy donors and PRF exudates were collected on the 7th day of incubation. hPDLCs were obtained from healthy premolars, cultured by a tissue explant method and identified with anti‑vimentin and anti‑cytokeratin antibodies. PRF exudates were added to hPDLCs in different concentrations to evaluate cell proliferation and osteogenic differentiation. The proliferation of hPDLCs was measured using a colorimetric assay. Osteogenic differentiation and mineralization were determined by Alizarin red staining, alkaline phosphatase activity (ALP), western blotting and reverse transcription‑quantitative polymerase chain reaction. Cell proliferation was enhanced by addition of the PRF exudate, which also promoted the formation of mineralized matrix nodules and upregulated ALP activity and osteoblast‑associated levels of osteocalcin, runt‑related transcription factor and osterix gene expression. As these stimulatory effects occurred in a dose‑dependent manner, it was concluded that high concentrations of the PRF exudate served an essential role in the proliferation, osteogenic differentiation and mineralization of hPDLCs in vitro. The present study demonstrated that PRF exudate enhanced hPDLC proliferation, induced the osteoblastic differentiation of hPDLCs into mineralized tissue‑formation cells in vitro, and may therefore provide potential benefits for periodontal tissue engineering; contributing to the primary processes of periodontal tissue regeneration. From the perspective of both economics and biology, PRF has greater clinical benefits than analogous growth factors.
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November-2018
Volume 18 Issue 5

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Li X, Yang H, Zhang Z, Yan Z, Lv H, Zhang Y and Wu B: Platelet‑rich fibrin exudate promotes the proliferation and osteogenic differentiation of human periodontal ligament cells in vitro. Mol Med Rep 18: 4477-4485, 2018
APA
Li, X., Yang, H., Zhang, Z., Yan, Z., Lv, H., Zhang, Y., & Wu, B. (2018). Platelet‑rich fibrin exudate promotes the proliferation and osteogenic differentiation of human periodontal ligament cells in vitro. Molecular Medicine Reports, 18, 4477-4485. https://doi.org/10.3892/mmr.2018.9472
MLA
Li, X., Yang, H., Zhang, Z., Yan, Z., Lv, H., Zhang, Y., Wu, B."Platelet‑rich fibrin exudate promotes the proliferation and osteogenic differentiation of human periodontal ligament cells in vitro". Molecular Medicine Reports 18.5 (2018): 4477-4485.
Chicago
Li, X., Yang, H., Zhang, Z., Yan, Z., Lv, H., Zhang, Y., Wu, B."Platelet‑rich fibrin exudate promotes the proliferation and osteogenic differentiation of human periodontal ligament cells in vitro". Molecular Medicine Reports 18, no. 5 (2018): 4477-4485. https://doi.org/10.3892/mmr.2018.9472