Silencing of perilipin by short hairpin RNA inhibits proliferation and induces apoptosis in liposarcoma cells

  • Authors:
    • Ling‑Xin Meng
    • Yu‑Xiu Zheng
    • Mao‑Lei He
    • Xiao‑Ming Zhou
    • Shu‑Yan Sun
    • Zhao‑Jun Ding
    • Qin Meng
    • Bing‑Cheng Li
    • Yan‑Wei Sun
  • View Affiliations

  • Published online on: September 5, 2018     https://doi.org/10.3892/mmr.2018.9461
  • Pages: 4571-4576
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Abstract

Previous studies have identified that perilipin-1 (PLIN1) is a highly specific marker for liposarcoma. However, its functions have yet to be fully elucidated. The aim of the present study was to investigate the potential role of PLIN1 in the proliferation, migration and apoptosis of liposarcoma cells. Short hairpin RNA was designed to inhibit PLIN1 levels. Cell proliferation was monitored by Cell Counting Kit‑8 assay and cell migration determined by wound healing assay. Flow cytometry was performed to assess the cell cycle distributions and apoptosis in liposarcoma cells. The results demonstrated that the expression of PLIN1 was significantly upregulated in liposarcoma tumor tissues compared with normal adipose tissues. Silencing of PLIN1 by short hairpin RNA significantly inhibited proliferation and migration and induced G1 phase cell cycle arrest and apoptosis in liposarcoma cell lines. It was identified that PLIN1 serves a crucial role in the pathogenesis and progression of liposarcoma and may be a potential therapeutic target for its clinical management.
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November-2018
Volume 18 Issue 5

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Meng LX, Zheng YX, He ML, Zhou XM, Sun SY, Ding ZJ, Meng Q, Li BC and Sun YW: Silencing of perilipin by short hairpin RNA inhibits proliferation and induces apoptosis in liposarcoma cells. Mol Med Rep 18: 4571-4576, 2018.
APA
Meng, L., Zheng, Y., He, M., Zhou, X., Sun, S., Ding, Z. ... Sun, Y. (2018). Silencing of perilipin by short hairpin RNA inhibits proliferation and induces apoptosis in liposarcoma cells. Molecular Medicine Reports, 18, 4571-4576. https://doi.org/10.3892/mmr.2018.9461
MLA
Meng, L., Zheng, Y., He, M., Zhou, X., Sun, S., Ding, Z., Meng, Q., Li, B., Sun, Y."Silencing of perilipin by short hairpin RNA inhibits proliferation and induces apoptosis in liposarcoma cells". Molecular Medicine Reports 18.5 (2018): 4571-4576.
Chicago
Meng, L., Zheng, Y., He, M., Zhou, X., Sun, S., Ding, Z., Meng, Q., Li, B., Sun, Y."Silencing of perilipin by short hairpin RNA inhibits proliferation and induces apoptosis in liposarcoma cells". Molecular Medicine Reports 18, no. 5 (2018): 4571-4576. https://doi.org/10.3892/mmr.2018.9461