Therapeutic effects of a liquid bandage prepared with cellulose powders from Styela clava tunics and Broussonetia kazinoki bark: Healing of surgical wounds on the skin of Sprague Dawley rats

Park,Jin  Ju; Kim,Ji  Eun; Yun,Woo  Bin; Lee,Mi  Rim; Choi,Jun  Young; Song,Bo  Ram; Son,Hong  Joo; Lim,Yong; Kang,Hyun‑Gu; An,Beum  Soo; Yang,Seung  Yun; Seo,Sung  Baek; Hwang,Dae Youn

doi:10.3892/mmr.2018.9668

Therapeutic effects of a liquid bandage prepared with cellulose powders from Styela clava tunics and Broussonetia kazinoki bark: Healing of surgical wounds on the skin of Sprague Dawley rats

Authors:
- Jin Ju Park
- Ji Eun Kim
- Woo Bin Yun
- Mi Rim Lee
- Jun Young Choi
- Bo Ram Song
- Hong Joo Son
- Yong Lim
- Hyun‑Gu Kang
- Beum Soo An
- Seung Yun Yang
- Sung Baek Seo
- Dae Youn Hwang
View Affiliations

Affiliations: Department of Biomaterials Science, College of Natural Resources and Life Science/Life and Industry Convergence Research Institute, Pusan National University, Miryang, Gyeongsangnam‑do 50463, Republic of Korea, Department of Clinical Laboratory Science, College of Nursing and Healthcare Science, Dong‑Eui University, Busan 47340, Republic of Korea, Veterinary Medical Center and College of Veterinary Medicine, Chungbuk National University, Cheongju, North Chungcheong 28644, Republic of Korea
Published online on: November 19, 2018 https://doi.org/10.3892/mmr.2018.9668
Pages: 452-460
Copyright: © Park et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

Cellulose in different forms has extensively been applied in biomedical treatments, including scaffolding, tissue engineering and tissue formation. To evaluate the therapeutic effects of a liquid bandage (LB) prepared with cellulose powders from Styela clava tunics (SCT) and Broussonetia kazinoki bark (BSLB) for healing cutaneous wounds, the remedial effects of a low concentration (LoBSLB) and a high concentration (HiBSLB) of BSLB on skin regeneration and toxicity in Sprague Dawley rats. Results indicated that the total area of skin involved in the surgical wound was lower in the BSLB‑treated group compared with the Vehicle‑treated group at days 4‑12, although some variations were observed in the HiBSLB‑treated group. In addition, the BSLB‑treated group showed significantly enhanced width of the re‑epithelialization region and epidermal thickness when compared with the Vehicle‑treated group. Furthermore, significant stimulation in the expression level of collagen‑1 and the signaling pathway of VEGF after topical application of BSLB was indicated. No liver or kidney toxicities were detected for either doses of BSLB. Overall, the results of the present study suggest that BSLB accelerates the process of wound healing in surgical skin wounds of Sprague Dawley rats through stimulation of re‑epithelialization and connective tissue formation, without any accompanying significant toxicity.

View Figures

View References

1	Crépy L, Monchau F, Chai F, Raoul G, Hivart P, Hildebrand HF, Martin P and Joly N: Evaluation of a bio-based hydrophobic cellulose laurate film as biomaterial-study on biodegradation and cytocompatibility. J Biomed Mater Res B Appl Biomater. 100:1000–1008. 2012. View Article : Google Scholar : PubMed/NCBI
2	Lavoine N, Desloges I, Dufresne A and Bras J: Microfibrillated cellulose-its barrier properties and applications in cellulosic materials: A review. Carbohydr Polym. 90:735–764. 2012. View Article : Google Scholar : PubMed/NCBI
3	Spencer PC, Schmidt B, Samtleben W, Bosch T and Gurland HJ: Ex vivo model of hemodialysis membrane biocompatibility. Trans Am Soc Artif Intern Organs. 31:495–498. 1985.PubMed/NCBI
4	Gissinger D and Stamm A: A comparative study of cross-linked carboxymethylcellulose as tablet disintegrant. Pharm Ind. 42:189–192. 1980.
5	Franz G: Polysaccharides in pharmacy. Adv Polymer Sci. 76:1–30. 1986. View Article : Google Scholar
6	Ito H, Shibata T, Miyamoto T, Noishiki Y and Inagaki H: Formation of polyelectrolyte complexes between cellulose derivatives and their blood compatibility. J Appl Polym Sci. 31:2491–2500. 1986. View Article : Google Scholar
7	Matasuzaki K, Yamamoto I, Sato T and Oshima R: Synthesis of water-soluble branched polysaccharides and their antitumor activity, 1. Branched polysaccharides from cellulose acetate. Macromol Chem Phys. 186:449–456. 1985. View Article : Google Scholar
8	Lee CG, Da Silva CA, Lee JY, Hartl D and Elias JA: Chitin regulation of immune responses: An old molecule with new roles. Curr Opin Immunol. 20:684–689. 2008. View Article : Google Scholar : PubMed/NCBI
9	Hirano S: Chitin biotechnology applications. Biotechnol Annu Rev. 2:237–258. 1996. View Article : Google Scholar : PubMed/NCBI
10	Mu X, Yu H, Zhang C, Chen X, Cheng Z, Bai R, Wu X, Yu Q, Wu C and Diao Y: Nano-porous nitrocellulose liquid bandage modulates cell and cytokine response and accelerates cutaneous wound healing in a mouse model. Carbohydr Polym. 136:618–629. 2016. View Article : Google Scholar : PubMed/NCBI
11	Fricain JC, Granja PL, Barbosa MA, de Jéso B, Barthe N and Baquey C: Cellulose phosphates as biomaterials. In vivo biocompatibility studies. Biomaterials. 23:971–980. 2002. View Article : Google Scholar : PubMed/NCBI
12	Martson M, Viljanto J, Hurme T, Laippala P and Saukko P: Is cellulose sponge degradable or stable as implantation material? An in vivo subcutaneous study in the rat. Biomaterials. 20:1989–1995. 1999. View Article : Google Scholar : PubMed/NCBI
13	Petkewich R: Liquid bandages. Chem Eng News. 86:612008. View Article : Google Scholar
14	Choi SJ, Lee JH, Lee YH, Hwang DY and Kim HD: Synthesis and properties of polyurethane-urea-based liquid bandage materials. J Appl Polym Sci. 121:3516–3524. 2011. View Article : Google Scholar
15	Shepler TR and Seiff SR: Use of isobutyl cyanoacrylate tissue adhesive to stabilize external eyelid weights in temporary treatment of facial palsies. Ophthalmic Plast Reconstr Surg. 17:169–173. 2001. View Article : Google Scholar : PubMed/NCBI
16	Taravella MJ and Chang CD: 2-Octyl cyanoacrylate medical adhesive in treatment of a corneal perforation. Cornea. 20:220–221. 2001. View Article : Google Scholar : PubMed/NCBI
17	Suhr MA, Günther M and Springer IN: Ptosis relief after botox injection using dermabond. Plast Reconstr Surg. 114:262–263. 2004. View Article : Google Scholar : PubMed/NCBI
18	Osaki TH, Osaki MH, Belfort R Jr, Osaki T, Sant'anna AE and Haraguchi DK: Management of progressive myopathic blepharoptosis with daily application of octyl-2-cyanoacrylate liquid bandage. Ophthalmic Plast Reconstr Surg. 25:264–266. 2009. View Article : Google Scholar : PubMed/NCBI
19	Osaki TH, Osaki MH and Osaki TH: Temporary management of involutional entropion with octyl-2-cyanoacrylate liquid bandage application. Arq Bras Oftalmol. 73:120–124. 2010. View Article : Google Scholar : PubMed/NCBI
20	Song SH, Kim JE, Lee YJ, Kwak MH, Sung GY, Kwon SH, Son HJ, Lee HS, Jung YJ and Hwang DY: Cellulose film regenerated from Styela clava tunics have biodegradability, toxicity and biocompatibility in the skin of SD rats. J Mater Sci Mater Med. 25:1519–1530. 2014. View Article : Google Scholar : PubMed/NCBI
21	Hwang DY, Chae KR, Kang TS, Hwang JH, Lim CH, Kang HK, Goo JS, Lee MR, Lim HJ, Min SH, et al: Alterations in behavior, amyloid beta-42, caspase-3, and Cox-2 in mutant PS2 transgenic mouse model of Alzheimer's disease. FASEB J. 16:805–813. 2002. View Article : Google Scholar : PubMed/NCBI
22	Eaglstein WH: Moist wound healing with occlusive dressings: A clinical focus. Dermatol Surg. 27:175–181. 2001. View Article : Google Scholar : PubMed/NCBI
23	Nissen NN and DiPietro LA: Angiogenic mediators in healing wounds. angiogenesis in Health and Disease. Gabor MR: Marcel Dekker Inc.; New York: pp. 417–427. 2000
24	Ferrara N: Vascular endothelial growth factor and the regulation of angiogenesis. Recent Prog Horm Res. 55:15–36. 2000.PubMed/NCBI
25	Ferrara N: Role of vascular endothelial growth factor in regulation of physiological angiogenesis. Am J Physiol Cell Physiol. 208:C1358–C1366. 2001. View Article : Google Scholar
26	Taub PJ, Silver L and Weinberg H: Plastic surgical perspectives on vascular endothelial growth factor as gene therapy for angiogenesis. Plast Reconstr Surg. 105:1034–1042. 2000. View Article : Google Scholar : PubMed/NCBI
27	Gerber HP, McMurtrey A, Kowalski J, Yan M, Keyt BA, Dixit V and Ferrara N: Vascular endothelial growth factor regulates endothelial cell survival through the phosphatidylinositol 3′-kinase/Akt signal transduction pathway. Requirement for Flk-1/KDR activation. J Biol Chem. 273:30336–30343. 1998. View Article : Google Scholar : PubMed/NCBI
28	Abid MR, Guo S, Minami T, Spokes KC, Ueki K, Skurk C, Walsh K and Aird WC: Vascular endothelial growth factor activates PI3K/Akt/forkhead signaling in endothelial cells. Arterioscler Thromb Vasc Biol. 24:294–300. 2004. View Article : Google Scholar : PubMed/NCBI
29	Zheng J, Wen Y, Song Y, Wang K, Chen DB and Magness RR: Activation of multiple signaling pathways is critical for fibroblast growth factor 2- and vascular endothelial growth factor-stimulated ovine fetoplacental endothelial cell proliferation. Biol Reprod. 78:143–150. 2008. View Article : Google Scholar : PubMed/NCBI
30	Frank S, Hübner G, Breier G, Longaker MT, Greenhalgh DG and Werner S: Regulation of vascular endothelial growth factor expression in cultured keratinocytes. Implications for normal and impaired wound healing. J Biol Chem. 270:12607–12613. 1995. View Article : Google Scholar : PubMed/NCBI
31	Kant V, Gopal A and Kumar D, Bag S, Kurade NP, Kumar A, Tandan SK and Kumar D: Topically applied substance P enhanced healing of open excision wound in rats. Eur J Pharmacol. 715:345–353. 2013. View Article : Google Scholar : PubMed/NCBI
32	Sun X, Cheng L, Zhu W, Hu C, Jin R, Sun B, Shi Y, Zhang Y and Cui W: Use of ginsenoside Rg3-loaded electrospun PLGA fibrous membranes as wound cover induces healing and inhibits hypertrophic scar formation of the skin. Colloids Surf B Biointerfaces. 115:61–70. 2014. View Article : Google Scholar : PubMed/NCBI
33	Mian M, Beghè F and Mian E: Collagen as a pharmacological approach in wound healing. Int J Tissue React. 14 Suppl:S1–S9. 1992.
34	Kwak MH, Go J, Kim JE, Lee YJ, Lee SH, Lee HS, Son HJ, Jung YJ and Hwang DY: Property and efficacy analysis of hydrocolloid membrane containing Styela clava tunic on the wound repair of skin in SD rats. Biomat Res. 17:91–101. 2013.