Association between quasispecies variants of hepatitis B virus, as detected by high‑throughput sequencing, and progression of advanced liver disease in Indonesian patients

  • Authors:
    • Wahyu Aristyaning Putri
    • Yoshihiko Yano
    • Laura Navika Yamani
    • Yujiao Liang
    • Yan Mardian
    • Takako Utsumi
    • Soetjipto
    • Maria Inge Lusida
    • Yoshitake Hayashi
  • View Affiliations

  • Published online on: May 16, 2019     https://doi.org/10.3892/mmr.2019.10250
  • Pages: 16-24
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Abstract

Mutations in the hepatitis B virus (HBV) X region and truncation of the preS2 region are well‑known to affect the progression of liver disease. Recently, it has been observed that an increasing number of S region quasispecies variants are associated with disease progression. However, few studies have analysed quasispecies of the whole genome using high‑throughput sequencing methods. Using high‑throughput sequencing, whole‑genome variations in 12 Indonesian patients infected with HBV (eight with advanced liver disease and four with chronic hepatitis) were examined. Variations with cut‑off values of ≥1% of the total viral population were investigated. It was revealed that within the four open reading frames, quasispecies variations of the S and X regions were higher in advanced liver diseases compared with in chronic hepatitis (S region: 89.53 vs. 50.69%, P=0.047; X region: 76.95 vs. 35.88%, P=0.044). Notably, the mutation frequencies in the basal core promoter, B cell epitope, RT Box G, RNAseH and small S region were greater in advanced liver disease. The proportion of quasispecies variants increased for the majority of the mutations, with the exception for W196* in the small S gene, during disease progression. The present study demonstrated that quasispecies in the S and X regions of the HBV genome changed during disease progression and were associated with advanced liver disease development in Indonesian patients with HBV.
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July-2019
Volume 20 Issue 1

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Putri WA, Yano Y, Yamani LN, Liang Y, Mardian Y, Utsumi T, Soetjipto , Lusida MI and Hayashi Y: Association between quasispecies variants of hepatitis B virus, as detected by high‑throughput sequencing, and progression of advanced liver disease in Indonesian patients. Mol Med Rep 20: 16-24, 2019
APA
Putri, W.A., Yano, Y., Yamani, L.N., Liang, Y., Mardian, Y., Utsumi, T. ... Hayashi, Y. (2019). Association between quasispecies variants of hepatitis B virus, as detected by high‑throughput sequencing, and progression of advanced liver disease in Indonesian patients. Molecular Medicine Reports, 20, 16-24. https://doi.org/10.3892/mmr.2019.10250
MLA
Putri, W. A., Yano, Y., Yamani, L. N., Liang, Y., Mardian, Y., Utsumi, T., Soetjipto, ., Lusida, M. I., Hayashi, Y."Association between quasispecies variants of hepatitis B virus, as detected by high‑throughput sequencing, and progression of advanced liver disease in Indonesian patients". Molecular Medicine Reports 20.1 (2019): 16-24.
Chicago
Putri, W. A., Yano, Y., Yamani, L. N., Liang, Y., Mardian, Y., Utsumi, T., Soetjipto, ., Lusida, M. I., Hayashi, Y."Association between quasispecies variants of hepatitis B virus, as detected by high‑throughput sequencing, and progression of advanced liver disease in Indonesian patients". Molecular Medicine Reports 20, no. 1 (2019): 16-24. https://doi.org/10.3892/mmr.2019.10250