Chondrogenesis of myoblasts in biodegradable poly-lactide-co-glycolide scaffolds

Gu,Yanglin; Chen,Peng; Yang,Yusheng; Shi,Keqin; Wang,Yubin; Zhu,Wenhui; Zhu,Guoxing

doi:10.3892/mmr.2012.1240

Chondrogenesis of myoblasts in biodegradable poly-lactide-co-glycolide scaffolds

Authors:
- Yanglin Gu
- Peng Chen
- Yusheng Yang
- Keqin Shi
- Yubin Wang
- Wenhui Zhu
- Guoxing Zhu
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Affiliations: Department of Orthopedics, Wuxi No. 2 People's Hospital, Jiangsu 214002, P.R. China, Department of Sports Medicine, Dongfang Hospital Affiliated to Tongji University, Shanghai 200120, P.R. China
Published online on: December 18, 2012 https://doi.org/10.3892/mmr.2012.1240
Pages: 1003-1009

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Abstract

Myoblasts are considered to be an alternative cell source for cell-based meniscal repair due to their multiple differentiation potentials. This study addresses the chondrogenic differentiation of myoblasts seeded into poly-lactide-co-glycolide (PLGA) scaffolds following implantation in a subcutaneous pocket of nude mice. Canine myoblasts isolated from a Beagle were expanded and seeded into PLGA scaffolds and cultured in cartilage-derived morphogenetic protein-2 (CDMP-2) and transforming growth factor-β1 (TGF-β1)-containing medium for 2 weeks in vitro. The constructs were implanted into a subcutaneous pocket of 24 combined immunodeficiency mice and harvested after 8 and 12 weeks, respectively. Hematoxylin and eosin staining of the sections of the engineered cartilage at 8 and 12 weeks revealed the regeneration of fibrocartilage. Immunohistochemical staining confirmed a similar distribution of collagen type Ⅱ in the engineered cartilage as the normal meniscus. At 12 weeks, expression of mRNAs for type Ⅰ collagen, type Ⅱ collagen and aggrecan was detected by RT-PCR. The compressive moduli of engineered cartilage reached 85.72% of the normal meniscus at 12 weeks, with a high level of glycosaminoglycan (GAG) content (no statistical difference from normal). Myoblast-seeded PLGA scaffolds express a stable chondrogenic phenotype in a heterotopic model of cartilage transplantation and represent a suitable tool for tissue engineering of cartilage.

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1	Hart R, Janecek M, Siska V, Kucera B and Stipcák V: Correlation of long-term clinical and radiological results after meniscectomies. Acta Chir Orthop Traumatol Cech. 72:304–307. 2005.PubMed/NCBI
2	van der Wal RJ, Thomassen BJ and van Arkel ER: Long-term clinical outcome of open meniscal allograft transplantation. Am J Sports Med. 37:2134–2139. 2009.PubMed/NCBI
3	Yamasaki T, Deie M, Shinomiya R, Yasunaga Y, Yanada S and Ochi M: Transplantation of meniscus regenerated by tissue engineering with a scaffold derived from a rat meniscus and mesenchymal stromal cells derived from rat bone marrow. Artif Organs. 32:519–524. 2008. View Article : Google Scholar
4	Webber RJ, York JL, Vanderschilden JL and Hough AJ Jr: An organ culture model for assaying wound repair of the fibrocartilaginous knee joint meniscus. Am J Sports Med. 17:393–400. 1989. View Article : Google Scholar : PubMed/NCBI
5	Kang SW, Son SM, Lee JS, Lee ES, Lee KY, Park SG, Park JH and Kim BS: Regeneration of whole meniscus using meniscal cells and polymer scaffolds in a rabbit total meniscectomy model. J Biomed Mater Res A. 78:659–671. 2006. View Article : Google Scholar
6	Ishida K, Kuroda R, Miwa M, Tabata Y, Hokugo A, Kawamoto T, Sasaki K, Doita M and Kurosaka M: The regenerative effects of platelet-rich plasma on meniscal cells in vitro and its in vivo application with biodegradable gelatin hydrogel. Tissue Eng. 13:1103–1112. 2007. View Article : Google Scholar : PubMed/NCBI
7	Peretti GM, Gill TJ, Xu JW, Randolph MA, Morse KR and Zaleske DJ: Cell-based therapy for meniscal repair: a large animal study. Am J Sports Med. 32:146–158. 2004. View Article : Google Scholar : PubMed/NCBI
8	Lu SH, Yang AH, Wei CF, Chiang HS and Chancellor MB: Multi-potent differentiation of human purified muscle-derived cells: potential for tissue regeneration. BJU Int. 105:1174–1180. 2009.PubMed/NCBI
9	Zhou G, Liu W, Cui L, Wang X, Liu T and Cao Y: Repair of porcine articular osteochondral defects in non-weightbearing areas with autologous bone marrow stromal cells. Tissue Eng. 12:3209–3221. 2006. View Article : Google Scholar : PubMed/NCBI
10	Zhu W, Wang Y, Qiu G and Chen B: Characterization of the purification and primary culture of adult canine myoblasts in vitro. Mol Med Report. 3:463–468. 2010.PubMed/NCBI
11	Gu YL and Wang YB: Treatment of meniscal injury: a current concept review. Chin J Traumatol. 13:370–376. 2010.PubMed/NCBI
12	Gu Y, Wang Y, Dai H, Lu L, Cheng Y and Zhu W: Chondrogenic differentiation of canine myoblasts induced by cartilage-derived morphogenetic protein-2 and transforming growth factor-β1 in vitro. Mol Med Report. 5:767–772. 2012.PubMed/NCBI
13	Asakura A, Komaki M and Rudnicki M: Muscle satellite cells are multipotential stem cells that exhibit myogenic, osteogenic and adipogenic differentiation. Differentiation. 68:245–253. 2001. View Article : Google Scholar
14	Matsushita T, Matsui N, Fujioka H, Kubo S, Kuroda R, Kurosaka M and Yoshiya S: Expression of transcription factor sox9 in rat L6 myoblastic cells. Connect Tissue Res. 45:164–173. 2004. View Article : Google Scholar : PubMed/NCBI
15	Goldring K, Partridge T and Watt D: Muscle stem cells. J Pathol. 197:457–467. 2002. View Article : Google Scholar : PubMed/NCBI
16	Day CS, Kasemkijwattana C, Menetrey J, Floyd SS Jr, Booth D, Moreland MS, Fu FH and Huard J: Myoblast-mediated gene transfer to the joint. J Orthop Res. 15:894–903. 1997. View Article : Google Scholar : PubMed/NCBI
17	Koning M, Harmsen MC, van Luyn MJ and Werker PM: Current opportunities and challenges in skeletal muscle tissue engineering. J Tissue Eng Regen Med. 3:407–415. 2009. View Article : Google Scholar : PubMed/NCBI
18	Singh D, Nayak V and Kumar A: Proliferation of myoblast skeletal cells on three-dimensional supermacroporous cryogels. Int J Biol Sci. 6:371–381. 2010. View Article : Google Scholar : PubMed/NCBI
19	Marsano A, Millward-Sadler SJ, Salter DM, Adesida A, Hardingham T, Tognana E, Kon E, Chiari-Grisar C, Nehrer S, Jakob M and Martin I: Differential cartilaginous tissue formation by human synovial membrane, fat pad, meniscus cells and articular chondrocytes. Osteoarthritis Cartilage. 15:48–58. 2007. View Article : Google Scholar : PubMed/NCBI
20	Stern-Straeter J, Bran G, Riedel F, Sauter A, Hörmann K and Goessler UR: Characterization of human myoblast cultures for tissue engineering. Int J Mol Med. 21:49–56. 2008.
21	Moran JM, Pazzano D and Bonassar LJ: Characterization of polylactic acid-polyglycolic acid composites for cartilage tissue engineering. Tissue Eng. 9:63–70. 2003. View Article : Google Scholar : PubMed/NCBI
22	Cui L, Wu Y, Cen L, Zhou H, Yin S, Liu G, Liu W and Cao Y: Repair of articular cartilage defect in non-weight bearing areas using adipose derived stem cells loaded polyglycolic acid mesh. Biomaterials. 30:2683–2693. 2009. View Article : Google Scholar : PubMed/NCBI
23	Zwingmann J, Mehlhorn AT, Südkamp N, Stark B, Dauner M and Schmal H: Chondrogenic differentiation of human articular chondrocytes differs in biodegradable PGA/PLA scaffolds. Tissue Eng. 13:2335–2343. 2007. View Article : Google Scholar : PubMed/NCBI