Inhibitory effect of RNA-mediated knockdown of zinc finger protein 91 pseudogene on pancreatic cancer cell growth and invasion

Huang,Weiyi; Li,Ning; Hu,Jiong; Wang,Lei

doi:10.3892/ol.2016.4794

Inhibitory effect of RNA-mediated knockdown of zinc finger protein 91 pseudogene on pancreatic cancer cell growth and invasion

Authors:
- Weiyi Huang
- Ning Li
- Jiong Hu
- Lei Wang
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Affiliations: Department of Oncology, Shanghai Jiaotong University Affiliated Shanghai General Hospital, Shanghai 200085, P.R. China
Published online on: June 28, 2016 https://doi.org/10.3892/ol.2016.4794
Pages: 1343-1348
Copyright: © Huang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Worldwide, human pancreatic cancer is a rare malignancy with a poor prognosis. Long non‑coding RNAs (lncRNAs) are known to have a crucial role in cancer occurrence and progression; however, the role of pseudogene-expressed lncRNAs, a major type of lncRNA, have not been thoroughly analyzed in cancer. Therefore, the present study focused on zinc finger protein 91 pseudogene (ZFP91-P). ZFP91-P expression was initially detected in two pancreatic cancer cell lines by reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) and the highest expression of ZFP91‑P was found in the BXPC‑3‑H cell line. Subsequently, BXPC‑3‑H cells were transfected with ZFP91‑P short hairpin RNA (shRNA) using a plasmid vector and termed shZFP91‑P. Cells transfected with negative control plasmid vector were termed shCon. MTT and Transwell assays were performed to analyze the proliferation and migration of BXPC‑3‑H cells, respectively, and western blotting was used to detect epithelial‑mesenchymal transition markers, including vimentin and β‑catenin. The present study showed that depletion of ZFP91‑P markedly decreased pancreatic cancer cell proliferation and inhibited cell migration capacity. In addition, the expression of β‑catenin increased while vimentin expression decreased. The current findings suggest that high expression of ZFP91‑P promotes the migration of BXPC‑3‑H cells and may be a novel marker for early diagnosis for pancreatic cancer.

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