Role of CD133+ cells in tongue squamous carcinomas: Characteristics of ‘stemness’ in vivo and in vitro

Wang,Kai; Zhou,Xiao‑Kang; Wu,Min; Kang,Fei‑Wu; Wang,Zuo‑Lin; Zhu,Yan

doi:10.3892/ol.2016.4719

Role of CD133+ cells in tongue squamous carcinomas: Characteristics of ‘stemness’ in vivo and in vitro

Authors:
- Kai Wang
- Xiao‑Kang Zhou
- Min Wu
- Fei‑Wu Kang
- Zuo‑Lin Wang
- Yan Zhu
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Affiliations: Department of Oral and Maxillofacial Surgery, School and Hospital of Stomatology, Tongji University, Shanghai Engineering Research Center of Tooth Restoration and Regeneration, Shanghai 200072, P.R. China
Published online on: June 15, 2016 https://doi.org/10.3892/ol.2016.4719
Pages: 863-870
Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The objective of the present study was to determine the ‘stemness’ characteristics of CD133+ cells (harvested from the squamous cell tongue carcinoma Tca-8113 cell line) in vitro and to observe the tumourigenicity of the CD133+ cells in the bodies of NOD/SCID mice. Single cells from the Tca‑8113 cell line were observed for multiplication capacity in vitro. The suspending and pelletizing phenomena of Tca‑8113 cells in vitro were also observed, and the expression of CD133 in squamous cell carcinoma of the tongue was measured. The CD133+ cells from the Tca‑8113 cell line were purified, and their multiplication capacity and differentiation potency were observed. The NOD/SCID mouse model was established, and the tumourigenicity of the CD133+ cells was determined. The Tca‑8113 cells were observed to emerge in the form of suspending tumour spheres in squamous cell carcinoma of the tongue. Monoplasts with sustainable multiplication capacity accounted for ~5.32% of the spheres, and 0.95% of the CD133+ cells were expressed in squamous cell carcinoma of the tongue, with stronger multiplication capacity and differentiation potency in vitro. Stronger tumourigenicity was also observed in the bodies of the NOD/SCID mice. CD133– cells exhibited a multiplication capacity to a certain extent. Overall, the CD133+ cells in squamous cell carcinoma of the tongue are characterised by relatively strong tumourigenicity capacity in vivo and in vitro. To a certain extent, these CD133+ cells demonstrate the characteristics of ‘stemness’.

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1	Choi IK and Yun CO: Recent developments in oncolytic adenovirus-based immunotherapeutic agents for use against metastatic cancers. Cancer Gene Ther. 20:70–76. 2013. View Article : Google Scholar : PubMed/NCBI
2	Heppner GH and Miller BE: Tumor heterogeneity: biological implications and therapeutic consequences. Cancer metastasis Rev. 2:5–23. 1983. View Article : Google Scholar : PubMed/NCBI
3	Al-Hajj M, Wicha MS, Benito-Hernandez A, Morrison SJ and Clarke MF: Prospective identification of tumorigenic breast cancer cells. Proc Natl Acad Sci USA. 100:3983–3988. 2003. View Article : Google Scholar : PubMed/NCBI
4	Singh SK, Clarke ID, Terasaki M, Bonn VE, Hawkins C, Squire J and Dirks PB: Identification of a cancer stem cell in human brain tumors. Cancer Res. 63:582l–5828. 2003.
5	O'Brien CA, Pollett A, Gallinger S and Dick JE: A human colon cancer cell capable of initiating tumor growth in immunodeficient mice. Nature. 445:106–110. 2007. View Article : Google Scholar : PubMed/NCBI
6	Hide T and Kuratsu J: Progress in the study of brain tumor stem cells as treatment target. Brain Nerve. 61:781–789. 2009.(In Japanese). PubMed/NCBI
7	Binello E and Germano IM: Targeting glioma stem cells: a novel framework for brain tumors. Cancer Sci. 102:1958–1966. 2011. View Article : Google Scholar : PubMed/NCBI
8	Liu G, Yuan X, Zeng Z, Tunici P, Ng H, Abdulkadir IR, Lu L, Irvin D, Black KL and Yu JS: Analysis of gene expression and chemoresistance of CDl33⁺ cancer stem cells in glioblastoma. Mol Cancer. 5:672006. View Article : Google Scholar : PubMed/NCBI
9	Wei XD, Zhou L, Cheng L and Tian J: Experimental investigation of CD133 as a putative marker of tumor-initiating cell in laryngeal carcinoma. Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 41:940–944. 2006.(In Chinese). PubMed/NCBI
10	Yuan X, Curtin J, Xiong Y, Liu G, Waschsmann-Hogiu S, Farkas DL, Black KL and Yu JS: Isolation of cancer stem cells from adult glioblastoma multiforme. Oncogene. 23:9392–9400. 2004. View Article : Google Scholar : PubMed/NCBI
11	Behbod F and Rosen JM: Will cancer stem cells provide new therapeutic targets? Carcinogenesis. 26:703–711. 2005. View Article : Google Scholar : PubMed/NCBI
12	Bonnet D and Dick JE: Human acute myeloid leukemia is organized as a hierarchy that originates from a primitive hematopoietic cell. Nat Med. 3:730–737. 1997. View Article : Google Scholar : PubMed/NCBI
13	Jordan CT: Cancer stem cell biology: From leukemia to solid tumors. Curr Opin Cell Biol. 16:708–712. 2004. View Article : Google Scholar : PubMed/NCBI
14	Ricci-Vitiani L, Lombardi DG, Pilozzi E, Biffoni M, Todaro M, Peschle C and De Maria R: Identification and expansion of human colon-cancer initiating cells. Nature. 445:111–115. 2007. View Article : Google Scholar : PubMed/NCBI
15	Collins AT, Berry PA, Hyde C, Stower MJ and Maitland NJ: Prospective identification of tumorigenic prostate cancer stem cells. Cancer Res. 65:10946–10951. 2005. View Article : Google Scholar : PubMed/NCBI
16	Suetsugu A, Nagaki M, Aoki H, Motohashi T, Kunisada T and Moriwaki H: Characterization of CD133⁺ hepatocellular carcinoma cells as cancer stem/progenitor cells. Biochem Biophys Res Commun. 351:820–824. 2006. View Article : Google Scholar : PubMed/NCBI
17	Ma S, Chan KW, Hu L, Lee TK, Wo JY, Ng IO, Zheng BJ and Guan XY: Identification and characterization of tumorigenic liver cancer stem/progenitor cells. Gastroenterology. 132:2542–2556. 2007. View Article : Google Scholar : PubMed/NCBI
18	Dalerba P, Cho RW and Clarke MF: Cancer stem cells: Models and concepts. Annu Rev Med. 58:267–284. 2007. View Article : Google Scholar : PubMed/NCBI
19	Clarke MF, Dick JE, Dirks PB, Eaves CJ, Jamieson CH, Jones DL, Visvader J, Weissman IL and Wahl GM: Cancer stem cells-perspectives on current status and future directions: AACR workshop on cancer stem cells. Cancer Res. 66:9339–9344. 2006. View Article : Google Scholar : PubMed/NCBI
20	Bar EE, Chaudhry A, Lin A, Fan X, Schreck K, Matsui W, Piccirillo S, Vescovi AL, DiMeco F, Olivi A and Eberhart CG: Cyclopamine-mediated hedgehog pathway inhibition depletes stem-like cancer cells in glioblastoma. Stem Cells. 25:2524–2533. 2007. View Article : Google Scholar : PubMed/NCBI
21	Rowehl RA, Burke S, Bialkowska AB, Pettet DW 3rd, Rowehl L, Li E, Antoniou E, Zhang Y, Bergamaschi R, Shroyer KR, et al: Establishment of highly tumorigenic human colorectal cancer cell line (CR4) with properties of putative cancer stem cells. PLoS One. 9:e990912014. View Article : Google Scholar : PubMed/NCBI
22	Dontu G, Al-Hajj M, Abdallah WM, Clarke MF and Wicha MS: Stem cells in normal breast development and breast cancer. Cell Prolif. 36(Suppl 1): S59–S72. 2003. View Article : Google Scholar
23	Patrawala L, Calhoun T, Schneider-Broussard R, Zhou J, Claypool K and Tang DG: Side population is enriched in tumorigenic, stem-like cancer cells, whereas ABCG2⁺ and ABCG2^– cancer cells are similarly tumorigenic. Cancer Res. 65:6207–6219. 2005. View Article : Google Scholar : PubMed/NCBI
24	Harper LJ, Piper K, Common J, Fortune F and Mackenzie IC: Stem cell patterns in cell lines derived from head and neck squamous cell carcinoma. J Oral Pathol Med. 36:594–603. 2007. View Article : Google Scholar : PubMed/NCBI
25	Nishi H, Nishimura S, Higashiura M, Ikeya N, Ohta H, Tsuji T, Nishimura M, Ohnishi S and Higashi H: A new method for histamine release from purified peripheral blood basophils using monoclonal antibody-coated magnetic beads. J Immunol Methods. 240:39–46. 2000. View Article : Google Scholar : PubMed/NCBI
26	Zou B, Ji P, Sun S and Qi X: Screen and analysis of differentially expressed genes related to stem-like cells in tongue squamous cell carcinoma Tca8113 cell line. Huaxi Kouqiang Yixue Zazhi. 30:458–462. 2012.(In Chinese). PubMed/NCBI
27	Beier D, Hau P, Proescholdt M, Lohmeier A, Wischhusen J, Oefner PJ, Aigner L, Brawanski A, Bogdahn U and Beier CP: CD133⁺ and CD133^– glioblastoma-derived cancer stem cells show differential growth characteristics and molecular profiles. Cancer Res. 67:4010–4015. 2007. View Article : Google Scholar : PubMed/NCBI
28	Taipale J and Beachy PA: The hedgehog and Wnt signalling pathways in cancer. Nature. 411:349–354. 2001. View Article : Google Scholar : PubMed/NCBI