Discovery of genes from feces correlated with colorectal cancer progression

  • Authors:
    • Chia‑Long Lee
    • Chi‑Jung Huang
    • Shung‑Haur Yang
    • Chun‑Chao Chang
    • Chi‑Cheng Huang
    • Chih‑Cheng Chien
    • Ruey‑Neng Yang
  • View Affiliations

  • Published online on: August 31, 2016     https://doi.org/10.3892/ol.2016.5069
  • Pages: 3378-3384
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Colorectal cancer (CRC) is considered to develop slowly via a progressive accumulation of genetic mutations. Markers of CRC may serve to provide the basis for decision‑making, and may assist in cancer prevention, detection and prognostic prediction. DNA and messenger (m)RNA molecules that are present in human feces faithfully represent CRC manifestations. In the present study, exogenous mouse cells verified the feasibility of total fecal RNA as a marker of CRC. Furthermore, five significant genes encoding solute carrier family 15, member 4 (SLC15A4), cluster of differentiation (CD)44, 3-oxoacid CoA-transferase 1 (OXCT1), placenta‑specific 8 (PLAC8) and growth arrest‑specific 2 (GAS2), which are differentially expressed in the feces of CRC patients, were verified in different CRC cell lines using quantitative polymerase chain reaction. The present study demonstrated that the mRNA level of SLC15A4 was increased in the majority of CRC cell lines evaluated (SW1116, LS123, Caco‑2 and T84). An increased level of CD44 mRNA was only detected in an early‑stage CRC cell line, SW1116, whereas OXCT1 was expressed at higher levels in the metastatic CRC cell line CC‑M3. In addition, two genes, PLAC8 and GAS2, were highly expressed in the recurrent CRC cell line SW620. Genes identified in the feces of CRC patients differed according to their clinical characteristics, and this differential expression was also detected in the corresponding CRC cell lines. In conclusion, feces represent a good marker of CRC and can be interpreted through the appropriate CRC cell lines.
View Figures
View References

Related Articles

Journal Cover

November-2016
Volume 12 Issue 5

Print ISSN: 1792-1074
Online ISSN:1792-1082

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Lee CL, Huang CJ, Yang SH, Chang CC, Huang CC, Chien CC and Yang RN: Discovery of genes from feces correlated with colorectal cancer progression. Oncol Lett 12: 3378-3384, 2016
APA
Lee, C., Huang, C., Yang, S., Chang, C., Huang, C., Chien, C., & Yang, R. (2016). Discovery of genes from feces correlated with colorectal cancer progression. Oncology Letters, 12, 3378-3384. https://doi.org/10.3892/ol.2016.5069
MLA
Lee, C., Huang, C., Yang, S., Chang, C., Huang, C., Chien, C., Yang, R."Discovery of genes from feces correlated with colorectal cancer progression". Oncology Letters 12.5 (2016): 3378-3384.
Chicago
Lee, C., Huang, C., Yang, S., Chang, C., Huang, C., Chien, C., Yang, R."Discovery of genes from feces correlated with colorectal cancer progression". Oncology Letters 12, no. 5 (2016): 3378-3384. https://doi.org/10.3892/ol.2016.5069