Globose, cystic olfactory ensheathing cell tumor: A case report and literature review

  • Authors:
    • Yaoling Liu
    • Minghai Wei
    • Kang Yang
    • Zeshi Tan
    • Xu Sun
    • Xinyu Li
    • Ningwei Che
    • Lan Luan
    • Guanyu Wang
    • Xiaofeng Wang
    • Yuqiang Sun
    • Jian Yin
  • View Affiliations

  • Published online on: September 14, 2016     https://doi.org/10.3892/ol.2016.5131
  • Pages: 3981-3986
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Abstract

Olfactory ensheathing cell tumor (OECT) is one of the most rare intracranial, extra-axial tumors located in the anterior cranial fossa. The present study reports a case of a 34‑year‑old female patient who presented with a history of hyposmia for 1 year, as well as a gradual dizziness and emotional lability for 2 months. Magnetic resonance imaging of the brain revealed a globose, well‑defined cystic mass at the midline of the anterior cranial fossa, which was confirmed as an OECT by histology and was completely resected by bifrontal craniotomy. According to the immunostaining results, the tumor was positive for vimentin and S100 protein, and negative for epithelial membrane antigen, glial fibrillary acidic protein and cluster of differentiation 57 (also known as Leu‑7). The presentation, imaging findings, histopathological examination and histogenesis of OECT are discussed in the present study, along with a literature review.

Introduction

Traditional theories have supported that schwannomas originate from the optic and olfactory nerve, which, however, is rather unlikely, since these two nerves do not have a Schwann cell layer (1). Olfactory ensheathing cell tumor (OECT) is considered to originate from OECs of the olfactory mucosa or bulb. Not until Yasuda et al reported a case of OECT in 2006 did researchers start to focus on this rare type of tumor (2). Despite its rarity, ~50 cases of subfrontal olfactory groove schwannoma (OGS) and 8 cases of OECT, with clinical and radiological features similar to those of meningiomas and neuroblastomas in the midline of the anterior cranial fossa, have been published worldwide to date (211). The biological behaviour of OECT is currently unknown; The previous cases reported a benign course and a short follow-up time (2,511). OECs resemble Schwann cells on light and electron microscopy; however, the presence of cluster of differentiation 57, also known as Leu-7, is considered to differentiate OGS from OECT (2). OECT may be treated surgically using the endoscopic transnasal approach or craniotomy. OECT has an extremely good prognosis, with a reported 2-year survival rate of 100% (2,511). The present study reports the ninth case of OECT, which included confusing radiographic features, and presents a review of the literature.

Case report

On March 10, 2014, a 34-year-old woman was admitted to The Second Affiliated Hospital of Dalian Medical University (Dalian, China) presenting with a history of hyposmia for 1 year, accompanied by a gradual dizziness and emotional lability for 2 months. No seizures, visual disorder, cerebrospinal fluid rhinorrhea or neurofibromatosis-related family history was recorded. Physical examination detected neither focal neurological deficits nor abnormal pigmentation of neurofibromatosis, with the exception of right hyposmia. Magnetic resonance imaging (MRI) scan (MR Signa 3.0T Excite; GE Healthcare Bio-Sciences, Pittsburgh, PA, USA) revealed a 3.0×3.0×3.1-cm extra-axial, globose, well-defined mass at the midline of the anterior cranial fossa, which deviated to the right. The tumor displayed homogeneous hypointensity on T1-weighted images, hyperintensity on T2-weighted images and isointensity on fluid-attenuated inversion recovery, and was shown to have caused brain parenchyma deformation without obvious peritumoral edema (Fig. 1A, B and C). Following the administration of intravenous gadolinium (Sigma-Aldrich, St. Louis, MO, USA), the tumor was heterogeneously enhanced and a peculiar membrane was observed inside the cystic wall. The tumor boundary appeared clear and smooth, while the main part was tightly connected to the endocranium (Fig. 1D and E).

The patient underwent bifrontal craniotomy. When the right frontal lobe was softly lifted, a grayish red tumor with a glistening appearance was observed to be attached to the olfactory groove. The tumor was located in the intradural, extra-axial space, attached to the right anterior part of the cribriform plate. A large volume of a clear, non-congealable yellow liquid was extracted from inside the tumor. In addition, the right olfactory tract could not be identified, and the left olfactory tract had been squeezed by the tumor. The tumor was completely resected, and when dissected, it appeared to contain fat, as it was soft and yellow (Fig. 1F), however subsequent pathology revealed that fat was not present. The cribriform plate protruded slightly towards the nasal cavity; however, the bone cortex was intact. Using a microscope (MT4000D; Meiji Seika Kaisha, Ltd., Tokyo, Japan), the tumor tissue was examined histologically and the findings described a tumor composed of spindle cells, with an eosinophilic protoplasm and tadpole-shaped nucleus. No tumor necrosis or blood vessel hyperplasia was observed (Fig. 2A). Immunostaining results revealed positivity for S100 protein (polyclonal rabbit anti-human S100 antibody; cat. no. ENT4197; 1:10; Elabscience Biotechnology Co., Ltd, Wuhan, China) (Fig. 2B) and vimentin (polyclonal rabbit anti-human antibody; cat. no. ENT4879; 1:10; Elabscience Biotechnology Co., Ltd.) (Fig. 2C), and negativity for epithelial membrane antigen (monoclonal rat anti-human antibody; cat. no. BM0042; 1:10; Boster Inc., Wuhan, China) (Fig. 2D), glial fibrillary acidic protein (polyclonal chicken anti-mouse cat. no. ab4674; 1:10; Abcam, Cambridge, MA, USA) (Fig. 2E) and Leu-7 (monoclonal mouse anti-human antibody; cat. no. ab187274; 1:10; Abcam) (Fig. 2F). The Ki-67 (polyclonal rabbit anti-human antibody; cat. no. EPP14636; 1:10; Elabscience Biotechnology Co., Ltd.) index was 1% (Fig. 2G); therefore, the final pathological diagnosis was OECT. The postoperative course of the patient was uneventful, without adjuvant radiation and chemotherapy. No evidence of tumor recurrence was observed during the 6-month radiographic follow-ups.

Discussion

A literature search for relevant publications was performed in the following databases: PubMed (www.ncbi.nlm.nih.gov/pubmed), Web of Science (http://isiknowledge.com), MEDLINE (http://ovidsp.ovid.com/), Excerpta Medica dataBASE (www.elsevier.com/solutions/embase-biomedical-research) and Chinese Biomedical Database (www.sinomed.ac.cn/zh/), using the keywords ‘olfactory ensheathing cell tumor’ and ‘olfactory groove schwannoma’ in titles and/or abstracts. The search identified 8 cases of OECT (Table I). A review of these 8 cases in addition to the present one revealed that the initial symptoms of the patients included hyposmia or anosmia (6/7 patients; 86%; initial symptoms were not reported in 2 cases), seizures (4/9 patients; 44%), emotional lability (2/9 patients; 22%), headache and dizziness (3/9 patients; 33%), visual impairment (1/9 patients; 11%) and foreign body sensation (1/9 patients; 11%). The studies did not mention whether the patients that suffered the seizures were suspected of having OECT. A possible explanation for the seizures is dualism, since OECT is an extra-axial tumor, which makes it less likely for patients to develop seizures and thus, OECT and seizures may be derived from two separate pathologies. The mean age of the 9 patients reviewed was 31.9 years, which was consistent with that of patients with OGS (32.7±14.0 years) (12), and a female predominance was observed (females, 67%; males, 33%). In addition, radiographic evaluation revealed that 2/9 OECTs (22%) were cystic, 3/9 (33%) cystic-solid and 4/9 (44%) solid. Of note, the proportion of septum in cystic tumors was 60% (n=3/5), which was quite different from the majority of the cystic OGSs. If further studies confirm this finding, it may serve as an important radiographic clue for the diagnosis of OECTs. Furthermore, 7/9 OECTs (78%) were heterogeneously enhanced, while 2/9 (22%) were homogeneously enhanced. Bone erosion was a rather common finding (6/9 cases; 67%), whereas none of the patients presented with peritumoral edema. Intraoperative findings revealed that, out of the 9 OECTs, 8 originated from the olfactory bulb or tract, and 1 originated from the olfactory mucosa (9).

Table I.

Summary of 9 reported cases of olfactory ensheathing cell tumor.

Table I.

Summary of 9 reported cases of olfactory ensheathing cell tumor.

CaseAuthor (year)Agea/genderClinical featuresOlfaction disorderTumor appearanceBone erosionSeptum (if cystic)EnhancementEdemaNeurof.Detection of olfactory nervesRef
1Yasuda et al (2006)31/FSeizuresRight hyposmiaRound, cystic-solidYesYesHeterogenousNoNoRight absent(2)
2Ippili et al (2009)42/MSeizuresNormalIrregular, solidNoN.A.HeterogenousNoNoLeft absent(5)
3Darie et al (2010)28/FSeizures, emotional labilityAnosmiaIrregular, cloudy, solidYesN.A.HeterogenousNoNoLeft adherent to tumor(6)
4Lin et al (2010)32/MSeizuresN.M.Round, solidNoN.A.HeterogenousNoNoLeft absent(7)
5Yamaguchi et al (2010)30/FHeadacheRight anosmiaRound, solidYesN.A.HomogenousNoN.M.Right absent(8)
6Ogino-Nishimura et al (2012)41/FHeadacheAnosmiaIrregular, cysticYesNoHeterogenousNoN.M.Both sides detected(9)
7Al-Ghanem et al (2013)49/MVisual impairmentHyposmiaRound, cystic-solidYesNoHomogenousNoN.M.N.M.(10)
8Qi et al (2015)45/FForeign body sensationN.M.Irregular, cysticNoYesHeterogenousNoNoN.M.(11)
9Liu et al (2016)34/FDizziness, emotional labilityRight hyposmiaRound, cystic-solidYesYesHeterogenousNoNoRight absentPresent

a Age reported in years. F, female; M, male; Neurof., neurofibromatosis; N.M. not mentioned; N.A., not applicable; Ref, reference.

OECs are specialized glial cells capable of migrating even in astrocyte-rich environments; they ensheathe olfactory axons in order to facilitate axonal growth. Due to these characteristics, OECs have attracted considerable attention (11,13). Notably, OECs resemble Schwann cells in their appearance on light and electron microscopy, and in the majority of immunohistochemical staining features. The differentiation between OGS and OECT is mainly based on the presence of Leu-7 (2,5,14). All the cases of OECT reviewed in the present study were negative for Leu-7 (2,511); however, in the majority of OGS cases, testing for Leu-7 was either not performed or not mentioned in the corresponding studies (4,12,15). Furthermore, information on the Leu-7 reactivity in OGS is limited; ~20% of the tumors that were considered to be schwannomas were negative for Leu-7 (9,16); therefore, certain reported cases of OGS could have originated from OECs rather than Schwann cells.

In the present case, the radiological manifestations of the OECT were confusing, as a dural tail sign was evident on enhanced MRI and bone sclerosis and calcification were identified on computed tomography, which are similar to those observed with OGM (9). The differential diagnosis of tumors involving the extra-axial anterior cranial fossa, with or without bulging into the ethmoid sinus, should include OGM, OGS, OECT, low-grade astrocytoma, olfactory neuroblastoma and metastatic tumors. In the present case, the extra-axial tumor with the clear boundary that was observed to be connected to the endocranium, could have potentially been misdiagnosed as a dural tail sign on sagittal view MRI following contrast-enhancement. The presence of bone scalloping, as well as the absence of bone sclerosis and calcification, may assist the differentiation between OECTs and meningiomas (17). By contrast, OGMs exhibit a propensity to spread into the paranasal sinuses, particularly in recurrent cases, without displaying evidence of calcification. Furthermore, multiple foci of T2-hypointense MRI signals (associated with microbleeds) may suggest schwannoma (5). Table II comprises a list of the main differences among OGM, OGS, OECT, low-grade astrocytoma, olfactory neuroblastoma and metastatic tumors, based on radiologic manifestations, immunostaining results and possible origin.

Table II.

List of main differences among OGM, OGS, OECT, ON, Ast and Met, based on the radiologic manifestations and immunostaining results of the present and previous reports.

Table II.

List of main differences among OGM, OGS, OECT, ON, Ast and Met, based on the radiologic manifestations and immunostaining results of the present and previous reports.

Typical radiologic manifestation (CT and MRI scans)Immunostaining results


TumorCalcificationTumor appearanceEnhancementEdemaOther manifestationsGFAPS100VimLeu-7EMAPossible origin
OGMMostlySolidHomogenousPartialDural tail sign, lobulation, tendency to spread into, the paranasal sinuses±++Arachnoidal cap cells
OGSRareCystic, solid Heterogenous/HomogenousPartial when giantThe olfactory groove is an apparent origin but it can extend to one side, bone erosion, lobulation+++Aberrant Schwann cells, Schwann cells located 0.5 mm beyond the olfactory bulb, meningeal branches of trigeminal nerves, anterior ethmoidal nerves, nerve plexus of dural vessels
OECTPartialCystic, solid Heterogenous/HomogenousRareIncomplete olfactory nerves, bone erosion++OECs originating from the olfactory mucosa and bulb
ONPartialSolid Heterogenous/HomogenousPartialMainly grow in the nasal cavity, infiltration, bone erosion+N.A.+ Sensory neurons of the olfactory mucosa involving the superior nasal cavity and cribriform plate (NSE+)
AstPartialCystic, solidHeterogenousPartialIntra-axial lesions, infiltration into the frontal lobe+++Neuroepithelial cells
MetRareCystic, solidHeterogenousMostly, obviousMultiple, small lesions, clear boundaryDepends on primary lesionsDepends on primary lesions

[i] CT, computed tomography; MRI, magnetic resonance imaging; OGM, olfactory groove meningioma; OGS, olfactory groove schwannoma; OECT, olfactory ensheathing cell tumor; ON, olfactory neuroblastoma; Ast, astrocytoma; Met, metastatic tumors; GFAP, glial fibrillary acidic protein; Vim, vimentin; EMA, epithelial membrane antigen; N.A., not applicable; NSE, neuron specific enolase.

In previous reports, the aforementioned conditions were misdiagnosed as each other preoperatively, due to their rarity and enigmatic origin. The embryonic development of OEC is olfactory placode-derived, while that of Schwann cells is neural crest-derived; therefore, both tumors possess a similar peripheral origin (1). The following five main origins have been reported for OGS to date: i) Aberrant Schwann cells; ii) Schwann cells located 0.5 mm beyond the olfactory bulb; iii) meningeal branches of trigeminal nerves; iv) nerve plexus of dural vessels; and v) anterior ethmoidal nerves (4). Glial cells originate from the central nervous system (CNS), however, OECT has been demonstrated to originate from OECs of the olfactory mucosa or bulb, which are part of the peripheral nervous system (PNS). Thus, OECs may be considered an intermediate glial cell type that originate in the CNS, but function in the PNS. Furthermore, olfactory neuroblastoma has been reported to originate from the sensory neurons of the olfactory mucosa, and to involve the superior nasal cavity or the cribriform plate, while OGM has been reported to originate from arachnoidal cap cells as a truism (7,18).

Although the reported cases experienced a benign course, the follow-up for these cases are too short, and the origin of OECT, its biological behavior and the necessity for adjuvant chemotherapy remains uncertain. In conclusion, according to the limited number of clinical cases of OECT reported in the literature thus far, total surgical removal of the tumor remains the suggested treatment option, and long-term follow-up appears to be required.

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Spandidos Publications style
Liu Y, Wei M, Yang K, Tan Z, Sun X, Li X, Che N, Luan L, Wang G, Wang X, Wang X, et al: Globose, cystic olfactory ensheathing cell tumor: A case report and literature review. Oncol Lett 12: 3981-3986, 2016.
APA
Liu, Y., Wei, M., Yang, K., Tan, Z., Sun, X., Li, X. ... Yin, J. (2016). Globose, cystic olfactory ensheathing cell tumor: A case report and literature review. Oncology Letters, 12, 3981-3986. https://doi.org/10.3892/ol.2016.5131
MLA
Liu, Y., Wei, M., Yang, K., Tan, Z., Sun, X., Li, X., Che, N., Luan, L., Wang, G., Wang, X., Sun, Y., Yin, J."Globose, cystic olfactory ensheathing cell tumor: A case report and literature review". Oncology Letters 12.5 (2016): 3981-3986.
Chicago
Liu, Y., Wei, M., Yang, K., Tan, Z., Sun, X., Li, X., Che, N., Luan, L., Wang, G., Wang, X., Sun, Y., Yin, J."Globose, cystic olfactory ensheathing cell tumor: A case report and literature review". Oncology Letters 12, no. 5 (2016): 3981-3986. https://doi.org/10.3892/ol.2016.5131