Resveratrol reverses P-glycoprotein-mediated multidrug resistance of U2OS/ADR cells by suppressing the activation of the NF-κB and p38 MAPK signaling pathways Corrigendum in /10.3892/ol.2024.14225

Zhang,Rui; Lu,Ming; Zhang,Zhen; Tian,Xiliang; Wang,Shouyu; Lv,Decheng

doi:10.3892/ol.2016.5136

Resveratrol reverses P-glycoprotein-mediated multidrug resistance of U2OS/ADR cells by suppressing the activation of the NF-κB and p38 MAPK signaling pathways

Corrigendum in: /10.3892/ol.2024.14225

Authors:
- Rui Zhang
- Ming Lu
- Zhen Zhang
- Xiliang Tian
- Shouyu Wang
- Decheng Lv
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Affiliations: Department of Orthopedics, The First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116011, P.R. China
Published online on: September 14, 2016 https://doi.org/10.3892/ol.2016.5136
Pages: 4147-4154

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Abstract

The present study aimed to investigate the reversal effect of resveratrol on the phenomenon of multidrug resistance in U2OS/adriamycin (ADR) cells and to clarify the molecular mechanisms. To examine the cell survival and half‑inhibitory concentration (IC50) of ADR in U2OS and U2OS/ADR cells, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was used. The accumulation of ADR in U2OS and U2OS/ADR cells was investigated by flow cytometry. Reverse transcription‑quantitative polymerase chain reaction and western blot analysis were used to detect the expression of multidrug resistance protein 1 (MDR1), P‑glycoprotein (P‑gp), p65 and p38. Compared with U2OS cells, the IC50 value of ADR was significantly increased in U2OS/ADR cells, which exhibited high levels of MDR1/P‑gp. However, resveratrol could drastically reduce the IC50 value of ADR and the expression of MDR1/P‑gp, and increased the accumulation of ADR in U2OS/ADR cells. In addition, the expression levels of p38 (phosphorylated) and p65 (acetylated and total) in U2OS/ADR cells were also significantly suppressed by resveratrol. These results suggested that the nuclear factor (NF)‑κB and p38 mitogen-activated protein kinase (MAPK) signaling pathways are correlated with ADR‑induced drug resistance in U2OS/ADR cells. Furthermore, resveratrol could downregulate the expression of MDR1/P‑gp and reverse the drug resistance phenomenon in U2OS/ADR cells partly at least by suppressing the activation of the NF‑κB and p38 MAPK signaling pathways.

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