Open Access

Polyphenolic compounds from Korean Lonicera japonica Thunb. induces apoptosis via AKT and caspase cascade activation in A549 cells

  • Authors:
    • Kwang Il Park
    • Hyeonsoo Park
    • Arulkumar Nagappan
    • Gyeong Eun Hong
    • Silvia Yumnam
    • Ho Jeong Lee
    • Eun Hee Kim
    • Won Sup Lee
    • Sung Chul Shin
    • Jin A Kim
    • Sang Joon Lee
    • Jin Yeul Ma
    • Taesun Min
    • Jeong Doo Heo
    • Gon Sup Kim
  • View Affiliations

  • Published online on: February 23, 2017     https://doi.org/10.3892/ol.2017.5771
  • Pages: 2521-2530
  • Copyright: © Park et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Lonicera japonica Thunb. (L. japonica T.) has historically been used in Korean herbal medicine due to its anticancer and protective effects on the respiratory system. In the present study, the polyphenolic compounds in L. japonica T. were investigated using high-performance liquid chromatography coupled with tandem mass spectrometry, and its anticancer effects on A549 non‑small‑cell lung cancer cells were studied. Polyphenolic compounds potentially inhibit A549 cells in a dose-dependent manner. Flow cytometry and western blot analysis demonstrated that polyphenolic compounds induce apoptosis by regulating the protein expression levels of caspases, poly‑(ADP‑ribose) polymerase and the B‑cell lymphoma‑2‑associated X-protein/B‑cell lymphoma‑extra large ratio. Furthermore, polyphenolic compounds inhibited mitochondrial membrane potential activity. Caspase‑3 activity was increased in a dose‑dependent manner and polyphenolic compounds inhibited the activation of protein kinase B by dephosphorylation. These results suggest that polyphenolic compounds in A549 cells indicate the anticancer activity through the induction of apoptosis.

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April-2017
Volume 13 Issue 4

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Park KI, Park H, Nagappan A, Hong GE, Yumnam S, Lee HJ, Kim EH, Lee WS, Shin SC, Kim JA, Kim JA, et al: Polyphenolic compounds from Korean Lonicera japonica Thunb. induces apoptosis via AKT and caspase cascade activation in A549 cells. Oncol Lett 13: 2521-2530, 2017.
APA
Park, K.I., Park, H., Nagappan, A., Hong, G.E., Yumnam, S., Lee, H.J. ... Kim, G.S. (2017). Polyphenolic compounds from Korean Lonicera japonica Thunb. induces apoptosis via AKT and caspase cascade activation in A549 cells. Oncology Letters, 13, 2521-2530. https://doi.org/10.3892/ol.2017.5771
MLA
Park, K. I., Park, H., Nagappan, A., Hong, G. E., Yumnam, S., Lee, H. J., Kim, E. H., Lee, W. S., Shin, S. C., Kim, J. A., Lee, S. J., Ma, J. Y., Min, T., Heo, J. D., Kim, G. S."Polyphenolic compounds from Korean Lonicera japonica Thunb. induces apoptosis via AKT and caspase cascade activation in A549 cells". Oncology Letters 13.4 (2017): 2521-2530.
Chicago
Park, K. I., Park, H., Nagappan, A., Hong, G. E., Yumnam, S., Lee, H. J., Kim, E. H., Lee, W. S., Shin, S. C., Kim, J. A., Lee, S. J., Ma, J. Y., Min, T., Heo, J. D., Kim, G. S."Polyphenolic compounds from Korean Lonicera japonica Thunb. induces apoptosis via AKT and caspase cascade activation in A549 cells". Oncology Letters 13, no. 4 (2017): 2521-2530. https://doi.org/10.3892/ol.2017.5771