Open Access

Atmospheric particulate matter2.5 promotes the migration and invasion of hepatocellular carcinoma cells

  • Authors:
    • Qian Zhang
    • Qiong Luo
    • Xueyu Yuan
    • Li Chai
    • Dan Li
    • Jianjun Liu
    • Zhongwei Lv
  • View Affiliations

  • Published online on: March 29, 2017     https://doi.org/10.3892/ol.2017.5947
  • Pages: 3445-3450
  • Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Epidemiological data has demonstrated that particulate matter (PM) with an aerodynamic diameter ≤ 2.5 µm (PM2.5) is associated with cancer incidence. However, the precise mechanisms underlying PM2.5‑mediated hepatocellular carcinoma cancer (HCC) migration and invasion remain unclear. The aim of the present study was to explore the response of the HCC cell lines HepG2 and HuH-7 to PM2.5 exposure. The results revealed that PM2.5 treatment promoted the migration and invasion of HCC cells, in addition to increasing protein levels of matrix metalloproteinase (MMP)‑13. Additionally, PM2.5 induced intracellular reactive oxygen species formation in HCC cells. Further investigation revealed that phosphorylation of RAC‑alpha serine/threonine‑protein kinase (AKT) increased in response to PM2.5 exposure in HCC cells, and the AKT antagonist LY294002 reduced PM2.5‑induced migration, invasion and MMP‑13 expression. In addition, the data from the present study demonstrated that high concentrations of PM2.5 decreased the proliferation of normal HL7702 hepatocyte cells and promoted apoptosis. These results indicate that the activation of AKT by PM2.5 results in MMP‑13 overexpression, and stimulates HCC cell migration and invasion. In conclusion, the results from the present study demonstrate that PM2.5 promotes HCC development and elucidate a potential underlying molecular mechanism for this effect.
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May-2017
Volume 13 Issue 5

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Zhang Q, Luo Q, Yuan X, Chai L, Li D, Liu J and Lv Z: Atmospheric particulate matter2.5 promotes the migration and invasion of hepatocellular carcinoma cells. Oncol Lett 13: 3445-3450, 2017
APA
Zhang, Q., Luo, Q., Yuan, X., Chai, L., Li, D., Liu, J., & Lv, Z. (2017). Atmospheric particulate matter2.5 promotes the migration and invasion of hepatocellular carcinoma cells. Oncology Letters, 13, 3445-3450. https://doi.org/10.3892/ol.2017.5947
MLA
Zhang, Q., Luo, Q., Yuan, X., Chai, L., Li, D., Liu, J., Lv, Z."Atmospheric particulate matter2.5 promotes the migration and invasion of hepatocellular carcinoma cells". Oncology Letters 13.5 (2017): 3445-3450.
Chicago
Zhang, Q., Luo, Q., Yuan, X., Chai, L., Li, D., Liu, J., Lv, Z."Atmospheric particulate matter2.5 promotes the migration and invasion of hepatocellular carcinoma cells". Oncology Letters 13, no. 5 (2017): 3445-3450. https://doi.org/10.3892/ol.2017.5947