Long non-coding RNA expression profiles in gallbladder carcinoma identified using microarray analysis

Wang,Jiwen; Liu,Han; Shen,Xiaokun; Wang,Yueqi; Zhang,Dexiang; Shen,Sheng; Suo,Tao; Pan,Hongtao; Ming,Yue; Ding,Kan; Liu,Houbao

doi:10.3892/ol.2017.5893

Long non-coding RNA expression profiles in gallbladder carcinoma identified using microarray analysis

Authors:
- Jiwen Wang
- Han Liu
- Xiaokun Shen
- Yueqi Wang
- Dexiang Zhang
- Sheng Shen
- Tao Suo
- Hongtao Pan
- Yue Ming
- Kan Ding
- Houbao Liu
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Affiliations: Department of General Surgery, Zhongshan Hospital, General Surgery Institute, Fudan University, Shanghai 200032, P.R. China, Department of Surgical Oncology, Taizhou Hospital, Wenzhou Medical University, Taizhou, Zhejiang 317000, P.R. China, Department of General Surgery, The Fifth People's Hospital of Shanghai, Fudan University, Shanghai 200240, P.R. China, PET‑CT Center, Cancer Hospital, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100021, P.R. China, Glycobiology and Glycochemistry Laboratory, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, P.R. China
Published online on: March 22, 2017 https://doi.org/10.3892/ol.2017.5893
Pages: 3508-3516
Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Gallbladder carcinoma (GBC) is the most common biliary tract cancer and exhibits poor patient prognosis. Previous studies have identified that long non‑coding RNAs (lncRNAs) serve important regulatory roles in cancer biology. Alterations in lncRNAs are associated with several types of cancer. However, the contribution of lncRNAs to GBC remains unclear. To investigate the lncRNAs that are potentially involved in GBC, lncRNA profiles were identified in three pairs of human GBC and corresponding peri‑carcinomatous tissue samples using microarray analysis. Reverse transcription-quantitative polymerase chain reaction (RT‑qPCR) was used to validate the microarray data. In order to elucidate potential functions, Gene Ontology, Kyoto Encyclopedia of Genes and Genomes analysis, and network analysis were used to determine relevant signaling pathways. Abundant RNA probes were used, and 1,758 lncRNAs and 1,254 mRNAs were detected to be differentially expressed by the microarray. Compared with para‑carcinoma tissue, numerous lncRNAs were markedly upregulated or downregulated in GBC. The results demonstrated that the lncRNAs that were downregulated in GBC were more numerous compared with the lncRNAs that were upregulated. Among them, RP11‑152P17.2‑006 was the most upregulated, whereas CTA-941F9.9 was the most downregulated. The RT-qPCR results were consistent with the microarray data. Pathway analysis indicated that five pathways corresponded to the differentially expressed transcripts. It was demonstrated that lncRNA expression in GBC was markedly altered, and a series of novel lncRNAs associated with GBC were identified. The results of the present study suggest that the functions of lncRNAs are important in GBC development and progression.

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