Association between chromosomal aberration of exfoliated bladder cells in the urine and oxidative stress in patients with bladder transitional cell carcinoma

Wang,Dong; Feng,Jia‑Fu; Yuan,Guang‑Ya; Yang,Yong‑Hong; Liu,Yun‑Shuang; Yang,Yu‑Wei

doi:10.3892/ol.2017.6128

Association between chromosomal aberration of exfoliated bladder cells in the urine and oxidative stress in patients with bladder transitional cell carcinoma

Authors:
- Dong Wang
- Jia‑Fu Feng
- Guang‑Ya Yuan
- Yong‑Hong Yang
- Yun‑Shuang Liu
- Yu‑Wei Yang
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Affiliations: Department of Oncology, Mianyang Central Hospital, Affiliated to Southwest Medical University, Mianyang, Sichuan 621000, P.R. China, Department of Clinical Laboratory, Mianyang Central Hospital, Affiliated to Southwest Medical University, Mianyang, Sichuan 621000, P.R. China, Department of Urology, Mianyang Central Hospital, Affiliated to Southwest Medical University, Mianyang, Sichuan 621000, P.R. China, Department of Pathology, Mianyang Central Hospital, Affiliated to Southwest Medical University, Mianyang, Sichuan 621000, P.R. China
Published online on: May 5, 2017 https://doi.org/10.3892/ol.2017.6128
Pages: 137-144
Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The aim of the current study was to investigate the chromosomal aberrations of exfoliated bladder cells in the urine and blood oxidative stress in patients with bladder transitional cell carcinoma (BTCC). A total of 40 healthy controls and 246 patients with BTCC were recruited. Abnormal levels of CSP3, CSP7, CSP17 and GLPp16 were detected by fluorescence in situ hybridization (FISH) in exfoliated bladder cells in the urine of patients with BTCC. Serum total oxidant status (TOS), total antioxidant status (TAS) and oxidative stress index (OSI) were measured. Significant differences were observed in the abnormal CSP3, CSP7, CSP17, GLPp16 signals and FISH positive rate between patients with BTCC and healthy controls (P<0.001). Serum TOS, TAS and OSI were also significantly different between the two groups (P<0.001). The clinical stage of BTCC was not associated with abnormal CSP3, CSP7, CSP17, GLPp16 or FISH positive rate and oxidative stress (P>0.05). A Gamma rank correlation analysis revealed an association between the pathological grade of BTCC with abnormal CSP3, CSP7 and CSP17 as well as FISH positive rate (P<0.001). In addition, the clinical stage of BTCC was associated with serum TOS, TAS and OSI (P<0.001). Evaluation of the association between chromosomal aberrations and oxidative stress revealed that abnormal CSP3, CSP7 and CSP17 were positively associated with serum TOS and OSI (P<0.001), abnormal CSP7 and CSP17 were negatively associated with serum TAS (P<0.001), but abnormal GLPp16 was not associated with serum TOS, TAS or OSI (P>0.05). Therefore, the chromosomal aberrations of exfoliated bladder cells in the urine are associated with blood oxidative stress in patients with BTCC, and these factors may contribute to the occurrence and development of BTCC.

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