Polymorphisms of ‑800G/A and +915G/C in TGF‑β1 gene and lung cancer susceptibility
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- Published online on: May 16, 2017 https://doi.org/10.3892/ol.2017.6173
- Pages: 733-736
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Copyright: © Di et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
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Abstract
We studied the relationship between the polymorphisms of ‑800G/A and +915G/C in transforming growth factor‑β1 (TGF‑β1) gene and lung cancer susceptibility. The sequence‑specific primer polymerase chain reaction (PCR‑SSP) technique was used to test 156 non‑small cell lung cancer (NSCLC) patients that were selected as the observation group and 156 patients with pneumonia and tuberculosis that were selected as the control group (age and gender 1:1 proximal matching principle) and the polymorphisms of the first exon ‑800G/A and +915G/C TGF‑β1 genes. The expression of TGF‑β1 levels in peripheral blood was detected using ELISA. The proportion of ‑800G/A gene AA subtype and A allelic gene in the observation group was significantly higher than that in the control group, while the proportion of +915G/C gene CC subtype and C allelic gene was also significantly higher than that in the control group (P<0.05). The cancer risk [odds ratio (OR)] of patients with A allelic gene in ‑800G/A gene was 4.8 (95% CI=2.563‑6.537, P<0.05), while the cancer risk (OR) of patients with C allelic gene in +915G/C gene was 4.7 (95% CI=2.317‑5.864, P<0.05). The serum TGF‑β1 expression levels of ‑800G/A gene AA subtype in the observation group was significantly higher than the GG type, GA type and the control group, while the TGF‑β1 level of +915G/C gene CC subtype was significantly higher than the GG type, GC type and the control group (P<0.05). Therefore, the polymorphisms of ‑800G/A and +915G/C in TGF‑β1 gene are closely related to the lung cancer susceptibility.
