Delineation of retroperitoneal metastatic lymph nodes in ovarian cancer with near‑infrared fluorescence imaging

Pu,Tao; Xiong,Liqin; Liu,Qiyu; Zhang,Minxing; Cai,Qingqing; Liu,Haiou; Sood,Anil   K.; Li,Guiling; Kang,Yu; Xu,Congjian

doi:10.3892/ol.2017.6521

Delineation of retroperitoneal metastatic lymph nodes in ovarian cancer with near‑infrared fluorescence imaging

Authors:
- Tao Pu
- Liqin Xiong
- Qiyu Liu
- Minxing Zhang
- Qingqing Cai
- Haiou Liu
- Anil K. Sood
- Guiling Li
- Yu Kang
- Congjian Xu
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Affiliations: Department of Obstetrics and Gynecology, Shanghai Medical School, Fudan University, Shanghai 200032, P.R. China, School of Biomedical Engineering, Med‑X Research Institute, Shanghai Jiao Tong University, Shanghai 200030, P.R. China, Shanghai Key Laboratory of Female Reproductive Endocrine Related Diseases, Shanghai 200011, P.R. China, Department of Gynecologic Oncology and Reproductive Medicine, Center for RNAi and Non‑Coding RNA, The University of Texas MD Anderson Cancer Center, Houston, Texas 77030, USA
Published online on: July 5, 2017 https://doi.org/10.3892/ol.2017.6521
Pages: 2869-2877
Copyright: © Pu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Lymph node metastasis occurs in early‑stage and late‑stage ovarian cancers. Systematic lymphadenectomy is frequently conducted in an attempt to prevent disease progression. However, this method is associated with multiple complications. Therefore, it is necessary to develop a less invasive and more sensitive method for detecting lymphatic metastasis in ovarian cancer. The aim of the present study was to develop an appropriate fluorescent label for the analysis of lymphatic metastasis in vivo. To this end, epithelial ovarian cancer cells with high potential for lymph node metastasis were labeled using mCherry fluorescence. The cells were then imaged in vitro to determine the expression of mCherry, and in a mouse xenograft model in vivo. The data demonstrated the successful identification of metastatic retroperitoneal lymph nodes by co‑localization with lymph nodes labeled by near‑infrared fluorescence nanoparticles in vivo. These data provided important insights into the further development of methods for intra‑operative identification of lymphatic metastasis and the mechanisms underlying lymphatic metastasis.

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