The role of LASS2 in regulating bladder cancer cell tumorigenicity in a nude mouse model

Chen,Yujin; Wang,Haifeng; Xiong,Tao; Zou,Renchao; Tang,Zhaoran; Wang,Jiansong

doi:10.3892/ol.2017.6880

The role of LASS2 in regulating bladder cancer cell tumorigenicity in a nude mouse model

Authors:
- Yujin Chen
- Haifeng Wang
- Tao Xiong
- Renchao Zou
- Zhaoran Tang
- Jiansong Wang
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Affiliations: Department of Urology, The Second Affiliated Hospital of Kunming Medical University, Yunnan Institute of Urology, Kunming, Yunnan 650101, P.R. China, Department of Cadre Medical Care, The First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650031, P.R. China
Published online on: September 4, 2017 https://doi.org/10.3892/ol.2017.6880
Pages: 5149-5156
Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Previous in vitro studies have demonstrated that LAG1 longevity assurance homolog 2 (LASS2) is a novel tumor suppressor gene that is significantly associated with the proliferation and invasion ability of tumor cells. However, the role LASS2 serves in regulating bladder cancer cell tumorigenicity and tumor growth in vivo has not yet been elucidated in animal or clinical studies. In the present study, LASS2 knockdown in human bladder cancer EJ‑M3 cells significantly promoted the growth of xenografts in nude mice compared with the control group, while overexpression of LASS2 suppressed tumor growth; however, this was not statistically significant. Furthermore, LASS2 knockdown resulted in more apparent heteromorphism and a higher activity of matrix metalloproteinase (MMP)‑2 and MMP‑9 in xenograft tumors. The data from the present study demonstrated that LASS2 knockdown significantly promoted the tumorigenicity and growth of EJ‑M3 xenograft tumors in nude mice, and that LASS2 overexpression has a tendency to inhibit the growth of xenografts, suggesting that it may be a potential therapeutic target for bladder cancer.

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