Knockdown of MSI1 inhibited the cell proliferation of human osteosarcoma cells by targeting p21 and p27

Niu,Jianbing; Zhao,Xiulian; Liu,Qingsheng; Yang,Jinsan

doi:10.3892/ol.2017.6870

Knockdown of MSI1 inhibited the cell proliferation of human osteosarcoma cells by targeting p21 and p27

Authors:
- Jianbing Niu
- Xiulian Zhao
- Qingsheng Liu
- Jinsan Yang
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Affiliations: Department of Bone and Joint Surgery, Shandong Jining No. 1 People's Hospital, Jining, Shandong 272011, P.R. China, Department of Kidney and Chinese Medicine, Shandong Jinxiang County People's Hospital, Jinxiang, Shandong 272200, P.R. China
Published online on: September 1, 2017 https://doi.org/10.3892/ol.2017.6870
Pages: 5271-5278
Copyright: © Niu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Osteosarcoma is the most common type of primary bone cancer in children and adolescents, but its mechanism remains unclear. Musashi RNA‑binding protein 1 (MSI1) is highly expressed in certain cancer types and functions as a putative progenitor/stem cell marker. In the present study, it was demonstrated that MSI1 expression in osteosarcoma tissue was higher compared with in the paraneoplastic tissue samples. Knockdown of MSI1 using shRNA in MG‑63 and HOS cells inhibited cell proliferation in vitro and tumor formation in vivo, suggesting that MSI1 serves an essential role in osteosarcomagenesis. Further investigations demonstrated that the knockdown of MSI1 leads to the cell cycle arrest at G0/G1 phase, and the upregulation of p21 and p27 protein expression in osteosarcoma cells. Additionally, luciferase assays demonstrated that MSI1 can bind to the 3' untranslated regions of p21 and p27 mRNA. In conclusion, the results of the present study suggest that the knockdown of MSI11 can suppress cell proliferation of osteosarcoma by targeting p21 and p27 and subsequently inhibiting cell cycle progression.

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