Identification of a protein associated with the activity of cytokine‑induced killer cells

  • Authors:
    • Jingsong Cao
    • Cong Chen
    • Yongqiang Gao
    • Li Hu
    • Yu Liang
    • Jianhua Xiao
  • View Affiliations

  • Published online on: September 25, 2017     https://doi.org/10.3892/ol.2017.7042
  • Pages:6937-6942
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Abstract

Cytokine‑induced killer cells (CIKs) adoptive immunotherapy for efficient antitumor ability is used clinically, but details regarding the proteins associated with CIK activity remain unclear. In the current study, the cytotoxicity of CIKs on hepatoma was identified to be significantly downregulated by 1.61‑fold following gentamincin treatment. Further research revealed that a differentially expressed protein (P43) was significantly downregulated by 1.22‑fold using one‑dimensional gel electrophoresis analysis. Of these, the P43 was identified as human haptoglobin using liquid chromatography‑mass spectrometry. Western blotting demonstrated that the haptoglobin specifically reacted with rabbit anti‑human‑haptoglobin. Furthermore, western blotting results verified that the haptoglobin was significantly downregulated by 1.17‑fold compared with the control group. In addition, the expression of haptoglobin mRNA was significantly downregulated by 1.73‑fold following gentamincin treatment. Taken together, the results of the present study demonstrated that the expression of haptoglobin protein was associated with the activity of CIKs, and the results will be beneficial to the further investigation of CIK activity‑enhancement mechanism.

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December 2017
Volume 14 Issue 6

Print ISSN: 1792-1074
Online ISSN:1792-1082

2016 Impact Factor: 1.39
Ranked #68/217 Oncology
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APA
Cao, J., Chen, C., Gao, Y., Hu, L., Liang, Y., & Xiao, J. (2017). Identification of a protein associated with the activity of cytokine‑induced killer cells. Oncology Letters, 14, 6937-6942. https://doi.org/10.3892/ol.2017.7042
MLA
Cao, J., Chen, C., Gao, Y., Hu, L., Liang, Y., Xiao, J."Identification of a protein associated with the activity of cytokine‑induced killer cells". Oncology Letters 14.6 (2017): 6937-6942.
Chicago
Cao, J., Chen, C., Gao, Y., Hu, L., Liang, Y., Xiao, J."Identification of a protein associated with the activity of cytokine‑induced killer cells". Oncology Letters 14, no. 6 (2017): 6937-6942. https://doi.org/10.3892/ol.2017.7042