Open Access

Hypermethylation downregulates P2X7 receptor expression in astrocytoma

  • Authors:
    • Jing Liu
    • Ningning Li
    • Ruofan Sheng
    • Rui Wang
    • Zude Xu
    • Ying Mao
    • Yin Wang
    • Ying Liu
  • View Affiliations

  • Published online on: October 20, 2017     https://doi.org/10.3892/ol.2017.7241
  • Pages:7699-7704
  • Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The present study investigated the altered expression of p2X purinoceptor (P2X7R) in astrocytoma. Reverse transcription‑quantitative polymerase chain reaction and western blot analysis were used to determine the P2X7R expression in glioblastoma (GBM) and surrounding normal brain tissue. DNA methylation levels of P2X7R gene promoter in GBM were analyzed using a Sequenom MassARRAY® System. Immunohistochemistry (IHC) was used to detect the expression of P2X7R in astrocytoma at different malignancy grades, including diffuse astrocytoma, anaplastic astrocytoma and GBM. P2X7R mRNA and protein were significantly decreased in GBM compared with normal brain tissues. IHC results showed a negative correlation between P2X7R expression and tumor grade. The decreased P2X7R expression was mostly attributed to hypermethylation of its promoter. Therefore, P2X7R was found to perform an important role in tumorigenesis and progression of astrocytoma.

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December 2017
Volume 14 Issue 6

Print ISSN: 1792-1074
Online ISSN:1792-1082

2016 Impact Factor: 1.39
Ranked #68/217 Oncology
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APA
Liu, J., Li, N., Sheng, R., Wang, R., Xu, Z., Mao, Y. ... Liu, Y. (2017). Hypermethylation downregulates P2X7 receptor expression in astrocytoma. Oncology Letters, 14, 7699-7704. https://doi.org/10.3892/ol.2017.7241
MLA
Liu, J., Li, N., Sheng, R., Wang, R., Xu, Z., Mao, Y., Wang, Y., Liu, Y."Hypermethylation downregulates P2X7 receptor expression in astrocytoma". Oncology Letters 14.6 (2017): 7699-7704.
Chicago
Liu, J., Li, N., Sheng, R., Wang, R., Xu, Z., Mao, Y., Wang, Y., Liu, Y."Hypermethylation downregulates P2X7 receptor expression in astrocytoma". Oncology Letters 14, no. 6 (2017): 7699-7704. https://doi.org/10.3892/ol.2017.7241