Open Access

The hepatoprotective role of reduced glutathione and its underlying mechanism in oxaliplatin-induced acute liver injury

  • Authors:
    • Youzhi Lin
    • Yongqiang Li
    • Xiaohua Hu
    • Zhihui Liu
    • Jun Chen
    • Yulei Lu
    • Juan Liu
    • Sina Liao
    • Yumei Zhang
    • Rong Liang
    • Yan Lin
    • Qian Li
    • Caoyong Liang
    • Chunling Yuan
    • Xiaoli Liao
  • View Affiliations

  • Published online on: December 12, 2017     https://doi.org/10.3892/ol.2017.7594
  • Pages: 2266-2272
  • Copyright: © Lin et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Currently, the underlying mechanism of oxaliplatin (OXA) induced live injury is unclear. In addition, there is no standard clinical treatment for OXA‑induced acute liver injury (ALI). In this study, we established an animal model of OXA‑induced ALI, and studied the role of oxidative stress in OXA‑induced ALI and the impacts of reduced glutathione (GSH) treatment on OXA‑induced ALI. To establish an OXA‑induced ALI model, KM mice received intraperitoneal injection of OXA (8 mg/kg) for 4 days. Serum alanine aminotransferase (ALT), aspartate aminotransferase levels (AST), hepatic pathology and oxidative stress indicators in liver tissues were analyzed. To study the impact of GSH treatment on OXA‑induced ALI, mice were treated with GSH (400 mg/kg, i.p). In this ALI mouse model, ALT and AST levels were significantly increased (P<0.01). Liver pathological examination revealed varying degrees of liver cell turbidity and degeneration, even balloon‑like changes and focal necrosis, and sinusoidal hemorrhage in some cells. Compared with control group, the malondialdehyde (MDA) and GSH levels were significantly increased in OXA‑treated group (P<0.01), while the superoxide dismutase SOD and GSH‑peroxidase levels were decreased after OXA withdrawal (P<0.01). When GSH was used to treat OXA‑induced ALI mice, the pathological injury of liver tissues was alleviated, and serum ALT and AST were significantly decreased. In addition, GSH treatment could reduce the OXA‑induced increase of MDA level (P<0.05) in liver tissues, but had no impact on SOD level (P>0.05). We have successfully established an OXA‑induced ALI model. Using this model, we discover that oxidative stress plays an important role in OXA‑induced ALI. GSH‑based hepatoprotective therapy can partially inhibit oxidative stress and alleviate OXA‑induced ALI.

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February 2018
Volume 15 Issue 2

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Copy and paste a formatted citation
APA
Lin, Y., Li, Y., Hu, X., Liu, Z., Chen, J., Lu, Y. ... Liao, X. (2018). The hepatoprotective role of reduced glutathione and its underlying mechanism in oxaliplatin-induced acute liver injury. Oncology Letters, 15, 2266-2272. https://doi.org/10.3892/ol.2017.7594
MLA
Lin, Y., Li, Y., Hu, X., Liu, Z., Chen, J., Lu, Y., Liu, J., Liao, S., Zhang, Y., Liang, R., Lin, Y., Li, Q., Liang, C., Yuan, C., Liao, X."The hepatoprotective role of reduced glutathione and its underlying mechanism in oxaliplatin-induced acute liver injury". Oncology Letters 15.2 (2018): 2266-2272.
Chicago
Lin, Y., Li, Y., Hu, X., Liu, Z., Chen, J., Lu, Y., Liu, J., Liao, S., Zhang, Y., Liang, R., Lin, Y., Li, Q., Liang, C., Yuan, C., Liao, X."The hepatoprotective role of reduced glutathione and its underlying mechanism in oxaliplatin-induced acute liver injury". Oncology Letters 15, no. 2 (2018): 2266-2272. https://doi.org/10.3892/ol.2017.7594