Open Access

Genetic characterization and in vitro activity of antimicrobial combinations of multidrug-resistant Acinetobacter baumannii from a general hospital in China

  • Authors:
    • Fang Chen
    • Ling Wang
    • Min Wang
    • Yixin Xie
    • Xiaomeng Xia
    • Xianping Li
    • Yanhua Liu
    • Wei Cao
    • Tingting Zhang
    • Pengling Li
    • Min Yang
  • View Affiliations

  • Published online on: December 13, 2017     https://doi.org/10.3892/ol.2017.7600
  • Pages:2305-2315
  • Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: HTML 0 views | PDF 0 views     Cited By (CrossRef): 0 citations

Abstract

The present study aimed to develop a rational therapy based on the genetic epidemiology, molecular mechanism evaluation and in vitro antibiotic combinations activity in multidrug-resistant Acinetobacter baumannii (MDRAB). MDRAB was screened by the Kirby‑Bauer method. The random amplified polymorphic DNA technique was used to establish genetic fingerprinting, and a series of resistance genes were detected by polymerase chain reaction. Antimicrobial agents including amikacin (AK), cefoperazone/sulbactam (SCF I/II), meropenem (MEM), minocycline (MINO) and ciprofloxacin (CIP) were used to determine the minimum inhibitory concentrations (MICs) and interactions between antibiotics by the broth microdilution method and chequerboard assays. In total, 34 MDRAB strains were isolated and classified into 8 phenotypes A‑H, according to their general drug susceptibilities. A total of 4 major genotypes (I‑IV) were clustered at 60% a genotypic similarity threshold. High positive rates of β‑lactamase TEM‑1, topoisomerase IV, oxacillinase (OXA)‑23, AdeB family multidrug efflux RND transporter adeB, β‑lactamase AmpC, class 1 integrons (Int‑1), 16S rRNA methylase rmtA, phosphotransferase aph(3), 16S rRNA methyltransferase armA were presented to exceed 90%, acetylyltransferase aac(3)‑I, aac(6'‑I, ant(3'')‑I, 16S rRNA methylase rmtB, oxacillinase OXA‑24 and metallo‑β‑lactamase IMP‑5 genes demonstrated positive rates of 29.4‑85.29%, while adeRS two‑component system was not observed in any strain. MEM+SCF I or SCF II primarily exhibited synergistic effects. AK+SCF I, AK+SCF II, MINO+SCF I, MINO+SCF II, MINO+CIP and MINO+MEM primarily presented additive effects. AK+CIP demonstrated 70.59% antagonism. The antibacterial activity of SCF I was superior compared with that of SCF II. The results indicated the polyclonal genetic epidemiological trend of MDRAB in the Second Xiangya Hospital, and verified the complexity of genetic resistance. In addition, combinations suggested to be efficacious were MEM+SCF I and MEM+SCF II, which were more effective compared with other combinations for the management of MDRAB infection.

Related Articles

Journal Cover

February 2018
Volume 15 Issue 2

Print ISSN: 1792-1074
Online ISSN:1792-1082

2016 Impact Factor: 1.39
Ranked #68/217 Oncology
(total number of cites)

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
APA
Chen, F., Wang, L., Wang, M., Xie, Y., Xia, X., Li, X. ... Yang, M. (2018). Genetic characterization and in vitro activity of antimicrobial combinations of multidrug-resistant Acinetobacter baumannii from a general hospital in China. Oncology Letters, 15, 2305-2315. https://doi.org/10.3892/ol.2017.7600
MLA
Chen, F., Wang, L., Wang, M., Xie, Y., Xia, X., Li, X., Liu, Y., Cao, W., Zhang, T., Li, P., Yang, M."Genetic characterization and in vitro activity of antimicrobial combinations of multidrug-resistant Acinetobacter baumannii from a general hospital in China". Oncology Letters 15.2 (2018): 2305-2315.
Chicago
Chen, F., Wang, L., Wang, M., Xie, Y., Xia, X., Li, X., Liu, Y., Cao, W., Zhang, T., Li, P., Yang, M."Genetic characterization and in vitro activity of antimicrobial combinations of multidrug-resistant Acinetobacter baumannii from a general hospital in China". Oncology Letters 15, no. 2 (2018): 2305-2315. https://doi.org/10.3892/ol.2017.7600