Open Access

MicroRNA-196a-5p is a potential prognostic marker of delayed lymph node metastasis in early-stage tongue squamous cell carcinoma

  • Authors:
    • Tessho Maruyama
    • Kazuhide Nishihara
    • Masato Umikawa
    • Akira Arasaki
    • Toshiyuki Nakasone
    • Fumikazu Nimura
    • Akira Matayoshi
    • Kimiko Takei
    • Saori Nakachi
    • Ken‑Ichi Kariya
    • Naoki Yoshimi
  • View Affiliations

  • Published online on: December 8, 2017     https://doi.org/10.3892/ol.2017.7562
  • Pages: 2349-2363
  • Copyright: © Maruyama et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

MicroRNAs (miRs) are expected to serve as prognostic tools for cancer. However, many miRs have been reported as prognostic markers of recurrence or metastasis in oral squamous cell carcinoma patients. We aimed to determine the prognostic markers in early‑stage tongue squamous cell carcinoma (TSCC). Based on previous studies, we hypothesized that miR‑10a, 10b, 196a‑5p, 196a‑3p, and 196b were prognostic markers and we retrospectively performed miR expression analyses using formalin‑fixed paraffin‑embedded sections of surgical specimens. Total RNA was isolated from cancer tissues and adjacent normal tissue as control, and samples were collected by laser‑capture microdissection. After cDNA synthesis, reverse transcription‑quantitative polymerase chain reaction was performed. Statistical analyses for patient clinicopathological characteristics, recurrence/metastasis, and survival rates were performed to discern their relationships with miR expression levels, and the 2‑ΔΔCq method was used. miR‑196a‑5p levels were significantly upregulated in early‑stage TSCC, particularly in the lymph node metastasis (LNM) group. The LNM‑free survival rate in the low miR‑196a‑5p ΔΔCq value regulation group was found to be lower than that in the high ΔΔCq value regulation group (P=0.0079). Receiver operating characteristic analysis of ΔΔCq values revealed that miR‑196a‑5p had a P‑value=0.0025, area under the curve=0.740, and a cut‑off value=‑0.875 for distinguishing LNM. To our knowledge, this is the first study to examine LNM‑related miRs in early‑stage TSCC as well as miRs and ‘delayed LNM’ in head and neck cancer. miR‑196a‑5p upregulation may predict delayed LNM. Our data serve as a foundation for future studies to evaluate miR levels and facilitate the prediction of delayed LNM during early‑stage TSCC, which prevent metastasis when combined with close follow‑up and aggressive adjuvant therapy or elective neck dissection. Moreover, our data will serve as a foundation for future studies to evaluate whether miR‑196a‑5p can serve as a therapeutic marker for preventing metastasis.

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February 2018
Volume 15 Issue 2

Print ISSN: 1792-1074
Online ISSN:1792-1082

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APA
Maruyama, T., Nishihara, K., Umikawa, M., Arasaki, A., Nakasone, T., Nimura, F. ... Yoshimi, N. (2018). MicroRNA-196a-5p is a potential prognostic marker of delayed lymph node metastasis in early-stage tongue squamous cell carcinoma. Oncology Letters, 15, 2349-2363. https://doi.org/10.3892/ol.2017.7562
MLA
Maruyama, T., Nishihara, K., Umikawa, M., Arasaki, A., Nakasone, T., Nimura, F., Matayoshi, A., Takei, K., Nakachi, S., Kariya, K., Yoshimi, N."MicroRNA-196a-5p is a potential prognostic marker of delayed lymph node metastasis in early-stage tongue squamous cell carcinoma". Oncology Letters 15.2 (2018): 2349-2363.
Chicago
Maruyama, T., Nishihara, K., Umikawa, M., Arasaki, A., Nakasone, T., Nimura, F., Matayoshi, A., Takei, K., Nakachi, S., Kariya, K., Yoshimi, N."MicroRNA-196a-5p is a potential prognostic marker of delayed lymph node metastasis in early-stage tongue squamous cell carcinoma". Oncology Letters 15, no. 2 (2018): 2349-2363. https://doi.org/10.3892/ol.2017.7562